Significant enlargement of a specific subset of CD3 super(+)CD8 super(+) peripheral blood leukocytes mediating cytotoxic T-lymphocyte activity during human immunodeficiency virus infection

We have obtained a monoclonal antibody termed BY55 that defines an 80-kDa cell-surface structure on a subset of circulating peripheral blood mononucleocytes. This structure, which was not detected on most cell lines or activated lymphocytes, was expressed exclusively on 15-25% of CD2 super(+) circul...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1993-01, Vol.90 (20), p.9427-9430
Main Authors: Bensussan, A, Rabian, C, Schiavon, V, Bengoufa, D, Leca, G, Boumsell, L
Format: Article
Language:English
Subjects:
human immunodeficiency virus
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Summary:We have obtained a monoclonal antibody termed BY55 that defines an 80-kDa cell-surface structure on a subset of circulating peripheral blood mononucleocytes. This structure, which was not detected on most cell lines or activated lymphocytes, was expressed exclusively on 15-25% of CD2 super(+) circulating lymphocytes, including a major subset within the CD3 super(-) and the T-cell receptor gamma delta super(+) lymphocytes and a small percentage of the CD3 super(+)CD8 super(+) cells. Moreover, we have shown that the BY55 molecule delineated the competent killer circulating lymphocytes. In the present report, additional two- and three-color immunofluorescence studies of peripheral blood lymphocytes were done to precisely determine BY55 expression within the T-cell population. We found that the percentage of total BY55 super(+) lymphocytes as well as the percentage of CD3 super(+)CD8 super(+)BY55 super(+) was significantly increased in peripheral blood lymphocytes of human immunodeficiency virus-seropositive individuals.
ISSN:0027-8424