Diadenosine tetraphosphate improves adrenergic anti-glaucomatous drug delivery and efficiency

The effect of the dinucleotide P1, P4-Di (adenosine-5') tetraphosphate (Ap4A) in improving adrenergic anti-glaucomatous delivery by modifying the tight junction proteins of the corneal epithelium was evaluated. Stratified human corneal epithelial cells (HCLE) were treated with Ap4A (100 μM) for...

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Bibliographic Details
Published in:Experimental eye research 2015-05, Vol.134, p.141-147
Main Authors: Loma, Patricia, Guzman-Aranguez, Ana, Perez de Lara, Maria Jesus, Pintor, Jesus
Format: Article
Language:English
Subjects:
Adrenergic alpha-2 Receptor Agonists - administration & dosage
Adrenergic alpha-2 Receptor Agonists - pharmacokinetics
Adrenergic beta-Antagonists - administration & dosage
Adrenergic beta-Antagonists - pharmacokinetics
Animals
Antihypertensive Agents - administration & dosage
Antihypertensive Agents - pharmacokinetics
Aqueous Humor - metabolism
Blotting, Western
Brimonidine
Brimonidine Tartrate
Cell Membrane Permeability - drug effects
Cells, Cultured
Corneal epithelium
Diadenosine tetraphosphate
Dinucleoside Phosphates - pharmacology
Drug delivery
Drug Delivery Systems
Electric Impedance
Epithelium, Corneal - drug effects
Epithelium, Corneal - metabolism
Glaucoma
Glaucoma - drug therapy
Humans
Intraocular pressure
Intraocular Pressure - drug effects
Male
Occludin - metabolism
Quinoxalines - administration & dosage
Rabbits
Tight junction
Tight Junctions - drug effects
Tight Junctions - metabolism
Timolol
Timolol - administration & dosage
Zonula Occludens-1 Protein - metabolism
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Summary:The effect of the dinucleotide P1, P4-Di (adenosine-5') tetraphosphate (Ap4A) in improving adrenergic anti-glaucomatous delivery by modifying the tight junction proteins of the corneal epithelium was evaluated. Stratified human corneal epithelial cells (HCLE) were treated with Ap4A (100 μM) for 5 min and TJ protein levels and barrier function were analysed by western blotting and transepithelial electrical resistance (TEER), respectively. Western blot experiments showed a significant reduction at 2 h (45% reduction of ZO-1 and 65% reduction of occludin protein levels) as compared to non-treated (control) cells. Two hours after Ap4A treatment, TEER values were significantly reduced (65% as compared to control levels (p 
ISSN:0014-4835
1096-0007
DOI:10.1016/j.exer.2015.02.014