Anxiolytic Effects of Gly-His-Lys Peptide and Its Analogs

• Published in: Bulletin of experimental biology and medicine 2015-04, Vol.158 (6), p.726-728
• Main Authors: Bobyntsev, I. I., Chernysheva, O. I., Dolgintsev, M. E., Smakhtin, M. Yu, Belykh, A. E.
• Format: Article
• Language: English
• Subjects: Animals; Anti-Anxiety Agents - administration & dosage; Anti-Anxiety Agents - therapeutic use; Antianxiety agents; Anxiety - drug therapy; Biomedical and Life Sciences; Biomedicine; Cell Biology; Glycine; Internal Medicine; Laboratory Medicine; Lysine; Male; Maze Learning - drug effects; Oligopeptides - administration & dosage; Oligopeptides - therapeutic use; Pathology; Peptides; Rats
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-015-2847-3

Intraperitoneal administration of tripeptide Gly-His-Lys to male rats in doses of 0.5, 5, and 50 μg/kg 12 min before the start of the experiment produced an anxiolytic effect in the elevated plus maze test manifested in an increase in the time spent in open arms and shortened time spent in the closed arms. The anxiolytic effect was most pronounced after injection of 0.5 μg/kg peptide and decreased with increasing the dose of the peptide. Replacement of L-lysine with D-lysine in the tripeptide molecule was accompanied by a significant weakening of the neurotropic effects in all studied doses. Attachment of D-alanine to N- or C-terminus of Gly-His-Lys peptide leveled its anxiolytic action in all doses; significant changes in some measures of increased anxiety after administration at 50 μg/kg were found.