Immunochemical detection of Azospirilla in soil with genus-specific antibodies

Immunoelectrophoresis and immunodiffusion analysis with antibodies to whole intact cells of the type strain of nitrogen-fixing soil bacteria Azospirillum brasilense Sp7 revealed at least three conservative surface immunogenic proteins of azospirilla. Cross-reactions with these proteins made it possi...

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Bibliographic Details
Published in:Microbiology (New York) 2015-03, Vol.84 (2), p.263-267
Main Authors: Shirokov, A. A., Krasov, A. I., Selivanov, N. Yu, Burygin, G. L., Shchyogolev, S. Yu, Matora, L. Yu
Format: Article
Language:English
Subjects:
Azospirillum
Azospirillum brasilense
Biomedical and Life Sciences
Experimental Articles
Life Sciences
Medical Microbiology
Microbiology
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Summary:Immunoelectrophoresis and immunodiffusion analysis with antibodies to whole intact cells of the type strain of nitrogen-fixing soil bacteria Azospirillum brasilense Sp7 revealed at least three conservative surface immunogenic proteins of azospirilla. Cross-reactions with these proteins made it possible to use the above antibodies for the detection of azospirilla as a genus-specific probe, conjugated with horseradish peroxidase as an enzymatic label. Direct immune-enzyme analysis of soil suspensions (typical chernozem, Saratov oblast) confirmed applicability of the conjugates based on genus-specific antibodies to the surface proteins of azospirilla for direct detection of this bacterial genus in environmental samples. These results provide a basis for broad application of this method for the analysis of Azospirillum occurrence in soil.
ISSN:0026-2617
1608-3237
DOI:10.1134/S0026261715020137