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A single exposure to a sublethal pediocin concentration initiates a resistance-associated temporal cell envelope and general stress response in Listeria monocytogenes
Summary Listeria monocytogenes can cause the potentially fatal food‐borne disease listeriosis, and the use of bacteriocin‐producing lactic acid bacteria to control L. monocytogenes holds great promise. However, the development of bacteriocin resistance is a potential challenge, and the purpose of th...
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Published in: | Environmental microbiology 2015-04, Vol.17 (4), p.1134-1151 |
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creator | Laursen, Martin F. Bahl, Martin I. Licht, Tine R. Gram, Lone Knudsen, Gitte M. |
description | Summary
Listeria monocytogenes can cause the potentially fatal food‐borne disease listeriosis, and the use of bacteriocin‐producing lactic acid bacteria to control L. monocytogenes holds great promise. However, the development of bacteriocin resistance is a potential challenge, and the purpose of this study was to determine if exposure to sublethal concentrations of pediocin‐containing Lactobacillus plantarum WHE 92 supernatant could prime L. monocytogenes for resistance. By transcriptomic analysis, we found two, 55 and 539 genes differentially expressed after 10, 60 and 180 min of exposure to L. plantarum WHE 92 supernatant as compared with control exposures. We observed temporal expression changes in genes regulated by the two component system LisRK and the alternative sigma factors SigB and SigL. Additionally, several genes involved in bacteriocin resistance were induced. ΔlisR, ΔsigB and ΔsigL mutants were all more resistant than wild types to L. plantarum WHE 92 supernatant. Conclusively, LisRK, SigB and SigL regulation and genes associated with resistance are involved in the temporal adaptive response to pediocin, and all three regulatory systems affect pediocin resistance. Thus, a single exposure to a sublethal pediocin concentration initiates a response pointing to resistance, and indicates that further research exploring the link between adaptive responses and resistance is needed. |
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Listeria monocytogenes can cause the potentially fatal food‐borne disease listeriosis, and the use of bacteriocin‐producing lactic acid bacteria to control L. monocytogenes holds great promise. However, the development of bacteriocin resistance is a potential challenge, and the purpose of this study was to determine if exposure to sublethal concentrations of pediocin‐containing Lactobacillus plantarum WHE 92 supernatant could prime L. monocytogenes for resistance. By transcriptomic analysis, we found two, 55 and 539 genes differentially expressed after 10, 60 and 180 min of exposure to L. plantarum WHE 92 supernatant as compared with control exposures. We observed temporal expression changes in genes regulated by the two component system LisRK and the alternative sigma factors SigB and SigL. Additionally, several genes involved in bacteriocin resistance were induced. ΔlisR, ΔsigB and ΔsigL mutants were all more resistant than wild types to L. plantarum WHE 92 supernatant. Conclusively, LisRK, SigB and SigL regulation and genes associated with resistance are involved in the temporal adaptive response to pediocin, and all three regulatory systems affect pediocin resistance. Thus, a single exposure to a sublethal pediocin concentration initiates a response pointing to resistance, and indicates that further research exploring the link between adaptive responses and resistance is needed.</description><identifier>ISSN: 1462-2912</identifier><identifier>EISSN: 1462-2920</identifier><identifier>DOI: 10.1111/1462-2920.12534</identifier><identifier>PMID: 24920558</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Adaptation, Physiological - genetics ; Bacteriocins - pharmacology ; Bacteriology ; Cell Membrane - metabolism ; Cell Wall - drug effects ; Cell Wall - genetics ; Drug Resistance, Bacterial - genetics ; Food safety ; Foodborne Diseases - microbiology ; Humans ; Lactobacillus ; Lactobacillus plantarum - genetics ; Lactobacillus plantarum - metabolism ; Listeria ; Listeria monocytogenes ; Listeria monocytogenes - drug effects ; Listeria monocytogenes - genetics ; Listeriosis - microbiology ; Pediocins ; Sigma Factor - genetics ; Sigma Factor - metabolism ; Stress, Physiological</subject><ispartof>Environmental microbiology, 2015-04, Vol.17 (4), p.1134-1151</ispartof><rights>2014 Society for Applied Microbiology and John Wiley & Sons Ltd</rights><rights>2014 Society for Applied Microbiology and John Wiley & Sons Ltd.</rights><rights>Copyright © 2015 Society for Applied Microbiology and John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24920558$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Laursen, Martin F.</creatorcontrib><creatorcontrib>Bahl, Martin I.</creatorcontrib><creatorcontrib>Licht, Tine R.</creatorcontrib><creatorcontrib>Gram, Lone</creatorcontrib><creatorcontrib>Knudsen, Gitte M.</creatorcontrib><title>A single exposure to a sublethal pediocin concentration initiates a resistance-associated temporal cell envelope and general stress response in Listeria monocytogenes</title><title>Environmental microbiology</title><addtitle>Environ Microbiol</addtitle><description>Summary
Listeria monocytogenes can cause the potentially fatal food‐borne disease listeriosis, and the use of bacteriocin‐producing lactic acid bacteria to control L. monocytogenes holds great promise. However, the development of bacteriocin resistance is a potential challenge, and the purpose of this study was to determine if exposure to sublethal concentrations of pediocin‐containing Lactobacillus plantarum WHE 92 supernatant could prime L. monocytogenes for resistance. By transcriptomic analysis, we found two, 55 and 539 genes differentially expressed after 10, 60 and 180 min of exposure to L. plantarum WHE 92 supernatant as compared with control exposures. We observed temporal expression changes in genes regulated by the two component system LisRK and the alternative sigma factors SigB and SigL. Additionally, several genes involved in bacteriocin resistance were induced. ΔlisR, ΔsigB and ΔsigL mutants were all more resistant than wild types to L. plantarum WHE 92 supernatant. Conclusively, LisRK, SigB and SigL regulation and genes associated with resistance are involved in the temporal adaptive response to pediocin, and all three regulatory systems affect pediocin resistance. Thus, a single exposure to a sublethal pediocin concentration initiates a response pointing to resistance, and indicates that further research exploring the link between adaptive responses and resistance is needed.</description><subject>Adaptation, Physiological - genetics</subject><subject>Bacteriocins - pharmacology</subject><subject>Bacteriology</subject><subject>Cell Membrane - metabolism</subject><subject>Cell Wall - drug effects</subject><subject>Cell Wall - genetics</subject><subject>Drug Resistance, Bacterial - genetics</subject><subject>Food safety</subject><subject>Foodborne Diseases - microbiology</subject><subject>Humans</subject><subject>Lactobacillus</subject><subject>Lactobacillus plantarum - genetics</subject><subject>Lactobacillus plantarum - metabolism</subject><subject>Listeria</subject><subject>Listeria monocytogenes</subject><subject>Listeria monocytogenes - drug effects</subject><subject>Listeria monocytogenes - genetics</subject><subject>Listeriosis - microbiology</subject><subject>Pediocins</subject><subject>Sigma Factor - genetics</subject><subject>Sigma Factor - metabolism</subject><subject>Stress, Physiological</subject><issn>1462-2912</issn><issn>1462-2920</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqNkstu1TAQhi0EoqWwZocssWETsB07cZZV1Zt0uCyKkNhYjjMtLokdbAd6XojnZNJTzoIV3tgz8_2_bM8Q8pKztxzXOy4bUYlOYChULR-Rw33m8f7MxQF5lvMtY7ytW_aUHAiJgFL6kPw-ptmHmxEo3M0xLwloidTSvPQjlG92pDMMPjofqIvBQSjJFh8D9cEXbwtkhBNkn4vFcmVzRhjzAy0wzTGhg4NxpBB-whhnoDYM9AYCrJVcUJpX_RxDBjSlG3SC5C2dYohuW-LK5ufkybUdM7x42I_I57PTq5OLavPx_PLkeFN5KRpZcS31dSsdCNHrTg1Q617VzPK26QauulZgDEw4PVhQg6i5YEw53g-6ddK5-oi82fnOKf5YIBcz-bze3waISza80UzWqtP8P9AW3TslJaKv_0Fv45ICPgSpptNCMamQevVALf0Eg5mTn2zamr_NQkDtgF9-hO2-zplZZ8Gs3TZr5839LJjT95f3B9RVO936tXd7nU3fTYMDocyXD-eGcf1JXeiv5qr-A-hHtx0</recordid><startdate>201504</startdate><enddate>201504</enddate><creator>Laursen, Martin F.</creator><creator>Bahl, Martin I.</creator><creator>Licht, Tine R.</creator><creator>Gram, Lone</creator><creator>Knudsen, Gitte M.</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QH</scope><scope>7QL</scope><scope>7ST</scope><scope>7T7</scope><scope>7TN</scope><scope>7U9</scope><scope>7UA</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>201504</creationdate><title>A single exposure to a sublethal pediocin concentration initiates a resistance-associated temporal cell envelope and general stress response in Listeria monocytogenes</title><author>Laursen, Martin F. ; Bahl, Martin I. ; Licht, Tine R. ; Gram, Lone ; Knudsen, Gitte M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i4264-1848f74ce22b895de38b530a1769d159728b5e02c8dae5d2312005c1bd87c4cc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adaptation, Physiological - genetics</topic><topic>Bacteriocins - pharmacology</topic><topic>Bacteriology</topic><topic>Cell Membrane - metabolism</topic><topic>Cell Wall - drug effects</topic><topic>Cell Wall - genetics</topic><topic>Drug Resistance, Bacterial - genetics</topic><topic>Food safety</topic><topic>Foodborne Diseases - microbiology</topic><topic>Humans</topic><topic>Lactobacillus</topic><topic>Lactobacillus plantarum - genetics</topic><topic>Lactobacillus plantarum - metabolism</topic><topic>Listeria</topic><topic>Listeria monocytogenes</topic><topic>Listeria monocytogenes - drug effects</topic><topic>Listeria monocytogenes - genetics</topic><topic>Listeriosis - microbiology</topic><topic>Pediocins</topic><topic>Sigma Factor - genetics</topic><topic>Sigma Factor - metabolism</topic><topic>Stress, Physiological</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Laursen, Martin F.</creatorcontrib><creatorcontrib>Bahl, Martin I.</creatorcontrib><creatorcontrib>Licht, Tine R.</creatorcontrib><creatorcontrib>Gram, Lone</creatorcontrib><creatorcontrib>Knudsen, Gitte M.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Aqualine</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Oceanic Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Environmental microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Laursen, Martin F.</au><au>Bahl, Martin I.</au><au>Licht, Tine R.</au><au>Gram, Lone</au><au>Knudsen, Gitte M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A single exposure to a sublethal pediocin concentration initiates a resistance-associated temporal cell envelope and general stress response in Listeria monocytogenes</atitle><jtitle>Environmental microbiology</jtitle><addtitle>Environ Microbiol</addtitle><date>2015-04</date><risdate>2015</risdate><volume>17</volume><issue>4</issue><spage>1134</spage><epage>1151</epage><pages>1134-1151</pages><issn>1462-2912</issn><eissn>1462-2920</eissn><abstract>Summary
Listeria monocytogenes can cause the potentially fatal food‐borne disease listeriosis, and the use of bacteriocin‐producing lactic acid bacteria to control L. monocytogenes holds great promise. However, the development of bacteriocin resistance is a potential challenge, and the purpose of this study was to determine if exposure to sublethal concentrations of pediocin‐containing Lactobacillus plantarum WHE 92 supernatant could prime L. monocytogenes for resistance. By transcriptomic analysis, we found two, 55 and 539 genes differentially expressed after 10, 60 and 180 min of exposure to L. plantarum WHE 92 supernatant as compared with control exposures. We observed temporal expression changes in genes regulated by the two component system LisRK and the alternative sigma factors SigB and SigL. Additionally, several genes involved in bacteriocin resistance were induced. ΔlisR, ΔsigB and ΔsigL mutants were all more resistant than wild types to L. plantarum WHE 92 supernatant. Conclusively, LisRK, SigB and SigL regulation and genes associated with resistance are involved in the temporal adaptive response to pediocin, and all three regulatory systems affect pediocin resistance. Thus, a single exposure to a sublethal pediocin concentration initiates a response pointing to resistance, and indicates that further research exploring the link between adaptive responses and resistance is needed.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>24920558</pmid><doi>10.1111/1462-2920.12534</doi><tpages>18</tpages></addata></record> |
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subjects | Adaptation, Physiological - genetics Bacteriocins - pharmacology Bacteriology Cell Membrane - metabolism Cell Wall - drug effects Cell Wall - genetics Drug Resistance, Bacterial - genetics Food safety Foodborne Diseases - microbiology Humans Lactobacillus Lactobacillus plantarum - genetics Lactobacillus plantarum - metabolism Listeria Listeria monocytogenes Listeria monocytogenes - drug effects Listeria monocytogenes - genetics Listeriosis - microbiology Pediocins Sigma Factor - genetics Sigma Factor - metabolism Stress, Physiological |
title | A single exposure to a sublethal pediocin concentration initiates a resistance-associated temporal cell envelope and general stress response in Listeria monocytogenes |
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