Host–pathogen interactome analysis of Chikungunya virus envelope proteins E1 and E2

The envelope proteins of Chikungunya virus (CHIKV) are known to play crucial roles in viral infection and spread. Although the role of envelope proteins in viral infection has been studied, the cellular interactors of these proteins are still elusive. In the present study, the ectodomains of CHIKV e...

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Bibliographic Details
Published in:Virus genes 2015-04, Vol.50 (2), p.200-209
Main Authors: Dudha, Namrata, Rana, Jyoti, Rajasekharan, Sreejith, Gabrani, Reema, Gupta, Amita, Chaudhary, Vijay Kumar, Gupta, Sanjay
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Chikungunya Fever - genetics
Chikungunya Fever - metabolism
Chikungunya Fever - virology
Chikungunya virus
Chikungunya virus - genetics
Chikungunya virus - metabolism
Comparative analysis
Computer applications
Host-Pathogen Interactions
Humans
Immune system
Medical Microbiology
Plant Sciences
Protein Binding
Receptors, Virus - genetics
Receptors, Virus - metabolism
Two-Hybrid System Techniques
Viral envelope proteins
Viral Envelope Proteins - genetics
Viral Envelope Proteins - metabolism
Virology
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Summary:The envelope proteins of Chikungunya virus (CHIKV) are known to play crucial roles in viral infection and spread. Although the role of envelope proteins in viral infection has been studied, the cellular interactors of these proteins are still elusive. In the present study, the ectodomains of CHIKV envelope proteins (E1 and E2) have been used for a high throughput yeast two-hybrid (Y2H) screening to identify the interacting host protein partners. Following a comparative analysis between the viral–host protein interaction data generated from Y2H and computational approach, five host proteins interacting with E1 and three host proteins interacting with E2 common to both datasets were identified. These associations were further verified independently by pull down and protein interaction ELISA. The identified interactions shed light on the possible cellular machinery that CHIKV might be employing during viral entry, trafficking, and evasion of immune system.
ISSN:0920-8569
1572-994X
DOI:10.1007/s11262-014-1161-x