Faecal shedding of antimicrobial-resistant Clostridium difficile strains by dogs

OBJECTIVES To longitudinally assess the shedding of antimicrobial resistant Clostridium difficile strains by clinically healthy dogs raised at breeding facilities. METHODS 18 puppies from three different litters (#1, 2 and 3) were sampled weekly from parturition to day 20–55 postpartum. Faecal sampl...

Full description

Saved in:
Bibliographic Details
Published in:Journal of small animal practice 2015-03, Vol.56 (3), p.190-195
Main Authors: Álvarez-Pérez, S., Blanco, J. L., Peláez, T., Lanzarot, M. P., Harmanus, C., Kuijper, E., García, M. E.
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - pharmacology
Antimicrobial agents
Asymptomatic Diseases
Bacterial Shedding
Clostridium difficile
Clostridium difficile - drug effects
Clostridium difficile - genetics
Dog Diseases - microbiology
Dogs
Drug Resistance, Bacterial - genetics
Enterocolitis, Pseudomembranous - drug therapy
Enterocolitis, Pseudomembranous - microbiology
Enterocolitis, Pseudomembranous - veterinary
Feces - microbiology
Female
Male
Medical research
Microbial Sensitivity Tests
Ribotyping - veterinary
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:OBJECTIVES To longitudinally assess the shedding of antimicrobial resistant Clostridium difficile strains by clinically healthy dogs raised at breeding facilities. METHODS 18 puppies from three different litters (#1, 2 and 3) were sampled weekly from parturition to day 20–55 postpartum. Faecal samples from the mothers of litters #2 and 3 were also available for analysis. Bacterial isolates were ribotyped, tested for in vitro antimicrobial susceptibility and further characterised. RESULTS C. difficile was recovered from all sampled animals of litters #1 and 2, and a third of puppies from litter #3, but marked differences in C. difficile recovery were detected in different age groups (0–100%). Recovered PCR ribotypes included 056 (22 isolates), 010 (6 isolates), 078 and 213 (2 isolates each), and 009 and 020 (1 isolate each). Different ribotypes were shed by four individual animals. Regardless of their origin and ribotype, all isolates demonstrated full resistance to levofloxacin. Additionally, all but one isolate (belonging to ribotype 078) were resistant to ertapenem, and all ribotype 010 isolates displayed high‐level resistance to clindamycin, clarithromycin and erythromycin. A single ribotype 078 isolate showed metronidazole heteroresistance. CLINICAL SIGNIFICANCE Healthy dogs can shed antimicrobial‐resistant C. difficile strains.
ISSN:0022-4510
1748-5827
DOI:10.1111/jsap.12311