Isolation and characterization of Flavobacterium columnare from freshwater ornamental goldfish Carassius auratus

Filamentous bacteria overlaying ulcerated area on the body surface were observed in the wet-mout preparation from a moribund goldfish with saddle back appearance. The causative agent was identified as Flavobacterium columnrae, on the basis of biochemical test, species-specific polymerase chain react...

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Bibliographic Details
Published in:Journal of environmental biology 2015-03, Vol.36 (2), p.433-439
Main Authors: Verma, Dev Kumar, Rathore, Gaurav, Pradhan, Pravata Kumar, Sood, Neeraj, Punia, Peyush
Format: Article
Language:English
Subjects:
Animals
Bacteria
Carassius auratus
Environmental science
Fish Diseases - microbiology
Flavobacteriaceae Infections - microbiology
Flavobacteriaceae Infections - veterinary
Flavobacterium
Flavobacterium - classification
Flavobacterium - genetics
Flavobacterium - isolation & purification
Flavobacterium columnare
Fresh Water
Goldfish
Labeo rohita
Phylogeny
RNA, Bacterial - genetics
RNA, Ribosomal, 16S - genetics
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Summary:Filamentous bacteria overlaying ulcerated area on the body surface were observed in the wet-mout preparation from a moribund goldfish with saddle back appearance. The causative agent was identified as Flavobacterium columnrae, on the basis of biochemical test, species-specific polymerase chain reaction (PCR) and sequencing of 16S rDNA gene with the universal bacterial primers. Furthermore, the strain (ING-1) attributed to genomovar II in 16S rDNA PCR-restriction fragment length polymorphism (PCR-RFLP) and sequence analysis. In phylogenetic analysis, the strain ING-1, produced typical columnaris disease symptoms in rohu (Labeo rohita) fingerlings within 10 days. This is a new record about molecular detection and identification of Flavobacterium columnare, occurring naturally on a new host Carassius auratusin India.
ISSN:0254-8704
2394-0379