Effectiveness of Linezolid, super( 127)I-Linezolid and super( 131)I-Linezolid Against Methicillin-Susceptible Staphylococcus Aureus by Time Kill Curve Methods

• Published in: Molecular imaging and radionuclide therapy 2015-02, Vol.24 (1), p.44-44
• Main Authors: Demiroglu, Hasan, Avcibasi, Ugur, Sakarya, Serhan, Uenak, Perihan
• Format: Article
• Language: English
• Subjects: Staphylococcus aureus
• ISSN: 2146-1414; 2147-1959

The aim of the study was to compare the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and minimum biofilm inhibitory concentration (MBIC) of LNZ, super( 127)I-LNZ and super( 131)I-LNZ against methicillin susceptible Staphylococcus aureus ATCC 35556 (MSSA) biofilms. LNZ radiolabeled with super( 131)I and cold labeling study with super( 127)I was performed. Radiolabeling and inactive labeling quality-control studies of LNZ were carried out by using Thin Layer Radiochromatography and High Pressure Liquid Chromatography. LNZ, super( 127)I-LNZ and super( 131)I-LNZ against biofilm-forming MSSA was investigated, using a twofold serial broth microtiter method, biofilm challenge, and bacterial count recovery. The binding yield was obtained to be about 86 plus or minus 2% for radiolabeled LNZ. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration for LNZ, super( 127)I-LNZ and super( 131)I-LNZ ranged from 1 to 2 mu g/mL respectively. In time-kill studies LNZ, super( 127)I-LNZ and super( 131)I-LNZ were bactericidal against staphylococci, producing greater than or equal to 3 Log10 decrease in viable counts within 6 h at 2xMIC.