A multiplex RT-PCR for simultaneous detection and identification of five viruses and two viroids infecting chrysanthemum

Pathogens causing significant economic losses in chrysanthemum include tomato aspermy virus (TAV), chrysanthemum virus B (CVB), cucumber mosaic virus (CMV), tobacco mosaic virus (TMV), potato virus Y (PVY), chrysanthemum stunt viroid (CSVd) and chrysanthemum chlorotic mottle viroid (CChMVd). A multi...

Full description

Saved in:
Bibliographic Details
Published in:Archives of virology 2015-05, Vol.160 (5), p.1145-1152
Main Authors: Zhao, Xiting, Liu, Xingliang, Ge, Beibei, Li, Mingjun, Hong, Bo
Format: Article
Language:English
Subjects:
Agricultural production
Biomedical and Life Sciences
Biomedicine
China
Chrysanthemum
Chrysanthemum - virology
Chrysanthemum stunt viroid
Chrysanthemum virus b
Cucumber mosaic virus
Cytomegalovirus
Flowers & plants
Infections
Infectious Diseases
Medical Microbiology
Methods
Molecular Diagnostic Techniques - methods
Multiplex Polymerase Chain Reaction - methods
Original Article
Pallets
Pathogens
Plant diseases
Plant Diseases - virology
Polymerase chain reaction
Potato virus Y
Prevalence
Reverse Transcriptase Polymerase Chain Reaction - methods
RNA Viruses - classification
RNA Viruses - genetics
RNA Viruses - isolation & purification
Tobacco mosaic virus
Tomato aspermy virus
Viral infections
Viroids - classification
Viroids - genetics
Viroids - isolation & purification
Virology
Virology - methods
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Pathogens causing significant economic losses in chrysanthemum include tomato aspermy virus (TAV), chrysanthemum virus B (CVB), cucumber mosaic virus (CMV), tobacco mosaic virus (TMV), potato virus Y (PVY), chrysanthemum stunt viroid (CSVd) and chrysanthemum chlorotic mottle viroid (CChMVd). A multiplex reverse transcription polymerase chain reaction (RT-PCR) method, using specific primer sets for each virus or viroid, was developed for simultaneous detection and differentiation of TAV, CVB, CMV, TMV, PVY, CChMVd, and CSVd. The RT-PCR method was validated by testing chrysanthemum samples collected from different regions of China. In this study, CVB, TAV, TMV, PVY, CSVd, CMV, and CChMVd were detected, respectively, in 24.7 %, 17.5 %, 4.4 %, 4.4 %, 2.9 %, 2.5 %, and 1.5 % of the samples tested. These results indicate that CVB and TAV (24.7 % and 17.5 %) are common, whereas CMV, TMV, CChMVd, CSVd, and PVY (all below 5 %) are less frequently encountered. This new multiplex RT-PCR method has potential to be used routinely in large-scale virus and viroid surveys.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-015-2360-z