Fibrin-based microsphere reservoirs for delivery of neurotrophic factors to the brain

The therapeutic potential of neurotrophic factors to modify neuronal dysfunctions is limited by their short half-life. A biomaterials-based intervention, which protects these factors and allows a controlled release, is required. Hollow fibrin microspheres were fabricated by charge manipulation using...

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Bibliographic Details
Published in:Nanomedicine (London, England) England), 2015-03, Vol.10 (5), p.765-783
Main Authors: Samal, Juhi, Hoban, Deirdre B, Naughton, Carol, Concannon, Ruth, Dowd, Eilis, Pandit, Abhay
Format: Article
Language:English
Subjects:
Alzheimer's disease
Analysis
Animals
bioactivity
Biocompatible Materials
Brain
Brain - drug effects
brain delivery
Care and treatment
Cell Membrane Permeability
Delayed-Action Preparations
Diagnosis
Drug Delivery Systems
Fibrin
Gene expression
hollow spheres
Mesenchymal Stromal Cells - metabolism
Microspheres
Nanomedicine
Nerve Growth Factors - administration & dosage
Neurites - drug effects
Neurons - drug effects
Neurons - ultrastructure
neurotrophic factors
Neurotrophins
Parkinson's disease
PC12 Cells
Rats
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Summary:The therapeutic potential of neurotrophic factors to modify neuronal dysfunctions is limited by their short half-life. A biomaterials-based intervention, which protects these factors and allows a controlled release, is required. Hollow fibrin microspheres were fabricated by charge manipulation using polystyrene templates and were loaded with NGF. Bioactivity of released NGF was demonstrated by neuronal outgrowth assay in PC-12 cells followed by assessment for NGF release and host response. Fibrin-based hollow spheres showed high loading efficiency (>80%). Neurotrophin encapsulation into the microspheres did not alter its bioactivity and controlled release of NGF was observed in the study. Fibrin hollow microspheres act as a suitable delivery platform for neurotrophic factors with tunable loading efficiency and maintaining their bioactive form after release .
ISSN:1743-5889
1748-6963
DOI:10.2217/nnm.14.221