Lipid peroxidation in rats intoxicated with 3-nitropropionic acid

• Published in: Toxicon (Oxford) 1995-03, Vol.33 (3), p.327-331
• Main Authors: Fu, Yi-Tong, He, Feng-Sheng, Zhang, Shou-Lin, Zhang, Jin-Son
• Format: Article
• Language: English
• Subjects: Administration, Oral; Animals; Arthrinium; Biological and medical sciences; Brain - drug effects; Brain - enzymology; Brain - metabolism; Dose-Response Relationship, Drug; Electron Spin Resonance Spectroscopy; Food toxicology; Free Radicals - adverse effects; Free Radicals - analysis; Glutathione Peroxidase - metabolism; In Vitro Techniques; Lipid Peroxidation - drug effects; Liver - drug effects; Liver - enzymology; Liver - metabolism; Male; Malondialdehyde - metabolism; Medical sciences; Microsomes, Liver - drug effects; Microsomes, Liver - enzymology; Microsomes, Liver - metabolism; Mitochondria, Liver - drug effects; Mitochondria, Liver - enzymology; Mitochondria, Liver - metabolism; Neurotoxins - administration & dosage; Neurotoxins - toxicity; Nitro Compounds; Plant Extracts - administration & dosage; Plant Extracts - toxicity; Propionates - administration & dosage; Propionates - toxicity; Rats; Rats, Wistar; Superoxide Dismutase - metabolism; Toxicology
• ISSN: 0041-0101; 1879-3150
• DOI: 10.1016/0041-0101(94)00173-6

Using an electron spin resonance technique, free radical signals were observed to be increased in liver of rats 15, 30 and 45 min after orally dosing with 80 mg/kg 3-nitropropionic acid (3-NPA). Concentrations of 3-NPA from 595 to 2380 mg/litre enhanced the formation of adrenochrome from adrenaline in mitochondria and microsome suspensions of liver and brain. The activities of Superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) as well as the content of malonidialdehyde (MDA) were significantly increased in liver of rats dosed with 80 mg/kg 3-NPA. There was also a cerebral increase of activity of SOD and content of MDA. These results suggest that 3-NPA is able to produce lipid peroxidation both in vivo and in vitro.