Deficiency of Long Pentraxin PTX3 Promoted Neointimal Hyperplasia after Vascular Injury

• Published in: Journal of Atherosclerosis and Thrombosis 2015/04/21, Vol.22(4), pp.372-378
• Main Authors: Ishino, Mitsunori, Shishido, Tetsuro, Suzuki, Satoshi, Katoh, Shigehiko, Sasaki, Toshiki, Funayama, Akira, Netsu, Shunsuke, Hasegawa, Hiromasa, Honda, Shintaro, Takahashi, Hiroki, Arimoto, Takanori, Miyashita, Takehiko, Miyamoto, Takuya, Watanabe, Tetsu, Takeishi, Yasuchika, Kubota, Isao
• Format: Article
• Language: English
• Subjects: Animals; C-Reactive Protein - physiology; Cell Movement; Cell Proliferation; Hyperplasia - etiology; Hyperplasia - metabolism; Hyperplasia - pathology; Inflammation; Macrophages - metabolism; Macrophages - pathology; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Muscle, Smooth, Vascular - metabolism; Muscle, Smooth, Vascular - pathology; Neointima - etiology; Neointima - metabolism; Neointima - pathology; Nerve Tissue Proteins - physiology; Pentraxin 3; Proliferation; Vascular remodeling; Vascular System Injuries - complications; Vascular System Injuries - metabolism; Vascular System Injuries - pathology
• ISSN: 1340-3478; 1880-3873
• DOI: 10.5551/jat.26740

Aim: Pentraxin 3 (PTX3) is a novel marker for the primary local activation of innate immunity and inflammatory responses. Although clinical and experimental evidence suggests that PTX3 is associated with atherosclerosis, the relationship between PTX3 and vascular remodeling after wall injury remains to be determined. We investigated the effects of PTX3 on neointimal hyperplasia following wire vascular injury. Methods: PTX3 systemic knockout (PTX3-KO) mice and wild-type littermate (WT) mice were subjected to wire-mediated endovascular injury. At four weeks after wire-mediated injury, the areas of neointimal and medial hyperplasia were evaluated. Results: The PTX3-KO mice exhibited higher hyperplasia/media ratios than the WT mice after wire injury, and the degree of Mac-3-positive macrophage accumulation was significantly higher in the PTX3-KO mice than in the WT mice. Furthermore, the PTX3-KO mice showed a much greater increase in the number of PCNA-stained cells in the vascular wall than that observed in the WT mice. Conclusions: A deficiency of PTX3 results in deteriorated neointimal hyperplasia after vascular injury via the effects of macrophage accumulation and vascular smooth muscle cell proliferation and migration.