Three polygalacturonases constitutively synthesized by Aspergillus alliaceus

Of three molecular forms of polygalacturonases synthesized by Aspergillus alliaceus on glucose media, two were exopolygalacturonases (exoPG1 and exoPG2) and one was an endopolygalacturonase (endoPG). Low-methoxylated beet pectin was the preferred substrate for the endoPG and exoPG2 whereas pectic ac...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 1995-05, Vol.11 (3), p.330-332
Main Authors: Mikhailova, R.V, Sapunova, L.I, Lobanok, A.G
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Aspergillus
Aspergillus alliaceus
Biological and medical sciences
biosynthesis
Biotechnology
characterization
enzyme activity
Enzyme engineering
enzyme polymorphism
Enzymes and enzyme inhibitors
Fermentation
Fundamental and applied biological sciences. Psychology
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
Hydrolases
Methods. Procedures. Technologies
Microbiology
Mycology
polygalacturonase
Production of selected enzymes
temperature
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Summary:Of three molecular forms of polygalacturonases synthesized by Aspergillus alliaceus on glucose media, two were exopolygalacturonases (exoPG1 and exoPG2) and one was an endopolygalacturonase (endoPG). Low-methoxylated beet pectin was the preferred substrate for the endoPG and exoPG2 whereas pectic acid was the optimal substrate for exoPG1. The highest activities of endoPG, exoPG1 and exoPG2 were at pH 5.5, 3.5, and 6.0 and at 35, 45 to 50 and 35°C, respectively. Disks of potato-tuber tissue were macerated by endoPG, but not by exoPG1 or exoPG2.
ISSN:0959-3993
1573-0972
DOI:10.1007/BF00367111