Expression profiling of major heat shock protein genes during different seasons in cattle (Bos indicus) and buffalo (Bubalus bubalis) under tropical climatic condition

Heat shock proteins consist of highly conserved stress proteins, expressed in response to stress and play crucial roles in environmental stress tolerance and adaptation. The present study was conducted to identify major types of genes under the HSP70 family and other HSPs and to evaluate their expre...

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Bibliographic Details
Published in:Journal of thermal biology 2015-07, Vol.51, p.55-64
Main Authors: Kumar, Anil, Ashraf, Syma, Goud, T. Sridhar, Grewal, Anita, Singh, S.V., Yadav, B.R., Upadhyay, R.C.
Format: Article
Language:English
Subjects:
Acclimatization - genetics
Animals
Buffaloes
Cattle
Heat shock protein genes
Heat-Shock Proteins - blood
Heat-Shock Proteins - genetics
Heat-Shock Proteins - metabolism
Heat-Shock Response - genetics
Murrah
PBMC
RNA, Messenger - metabolism
Sahiwal
Seasons
Tharparkar
Transcriptome
Tropical Climate
Zebu cattle
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Summary:Heat shock proteins consist of highly conserved stress proteins, expressed in response to stress and play crucial roles in environmental stress tolerance and adaptation. The present study was conducted to identify major types of genes under the HSP70 family and other HSPs and to evaluate their expression pattern in Sahiwal and Tharparkar breeds of zebu cattle (Bos indicus) and Murrah buffalo (Bubalus bubalis) with respect to different seasons. Quantitative real time polymerase chain reaction was performed to analyze the transcript variants of three HSP70 family genes (HSPA1A, HSPA1B, and HSPA8) and HSP10, HSP60, HSP90 and HSF1 in each breed. The major finding of this study was the higher abundance of all the studied HSP genes during summer and winter compared to spring season, but the magnitude of increase was higher during summer as compared to winter. HSPA1A and HSPA1B genes showed maximal induction (P
ISSN:0306-4565
1879-0992
DOI:10.1016/j.jtherbio.2015.03.006