Antioxidant activity of polysaccharide purified from Acanthopanax koreanum Nakai stems in vitro and in vivo zebrafish model

• Published in: Carbohydrate polymers 2015-08, Vol.127, p.38-46
• Main Authors: Kang, Min-Cheol, Kim, Seo-Young, Kim, Eun-A., Lee, Ji-Hyeok, Kim, Young-Sun, Yu, Seok-Kyu, Chae, Jun Byoung, Choe, In-Hu, Cho, Jin Hun, Jeon, You-Jin
• Format: Article
• Language: English
• Subjects: Acanthopanax koreanum Nakai; Animals; Antioxidant activity; Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; Araliaceae - chemistry; Cell Death - drug effects; Cells, Cultured; Cercopithecus aethiops; Enzyme-assistant extract; Free Radicals - antagonists & inhibitors; Hydrogen Peroxide - antagonists & inhibitors; Hydrogen Peroxide - pharmacology; Models, Animal; Oxidative stress; Oxidative Stress - drug effects; Plant Stems - chemistry; Polysaccharide; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Reactive Oxygen Species - metabolism; Vero Cells; Zebrafish
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2015.02.043

•This study was designed to assess in vitro and in vivo antioxidant effect of ASP.•We isolated the APS by enzymatic extraction technique.•APS has a protective effect against H2O2-induced oxidative stress in cell and zebrafish model. In this study, the antioxidative effects of a purified polysaccharide isolated from the stems of Acanthopanax koreanum Nakai (ASP) on hydrogen peroxide-induced oxidative stress was investigated both in vitro and in vivo using a zebrafish model. A. koreanum Nakai stem was hydrolyzed using five carbohydrases and five proteases for the enzyme-assistant extraction. Of the enzyme-assistant extracts, the Protamex extract exhibited the highest yield and a potent scavenging activity against free radicals. Ethanol-added separation and anion exchange chromatography were conducted to identify the active polysaccharide. The purified polysaccharide significantly scavenged hydrogen peroxide and reduced hydrogen peroxide-induced cell death in Vero cells and in zebrafish. The results reveal that ASP is a useful antioxidant polysaccharide and might be available for relevant industrial applications.