Marker-ion analysis for quantification of mucoadhesivity of microparticles in particle-retention assays

[Display omitted] The objective of the present work was to develop an improved method to quantify particle retention on mucosal tissue under dynamic flow conditions with simultaneous determination of drug dissolution. The principle was to dissolve the collected inert carrier material and quantify sp...

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Bibliographic Details
Published in:International journal of pharmaceutics 2015-06, Vol.487 (1-2), p.157-166
Main Authors: Preisig, Daniel, Weingartner, Michael, Varum, Felipe J.O., Bravo, Roberto, Alles, Rainer, Huwyler, Jörg, Puchkov, Maxim
Format: Article
Language:English
Subjects:
Animals
Bioadhesion
Calcium Carbonate - chemistry
Chemistry, Pharmaceutical
Colon - metabolism
Colon delivery
Drug Delivery Systems
In Vitro Techniques
Intestinal Mucosa
Ions
Marker-ion analysis
Microparticles
Mucoadhesion
Nanoparticles
Particle Size
Particle-retention assay
Solubility
Swine
Tissue Adhesives
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Summary:[Display omitted] The objective of the present work was to develop an improved method to quantify particle retention on mucosal tissue under dynamic flow conditions with simultaneous determination of drug dissolution. The principle was to dissolve the collected inert carrier material and quantify specific marker ions by reliable analytical methods. The mucoadhesive model particles consisted of drug-loaded porous calcium carbonate microcarriers coated with chitosan, and quantification of calcium ions by capillary electrophoresis enabled to determine particle-retention kinetics on colonic mucosal tissue. The method was validated by image analysis, and the particle-retention assay was successfully applied to granulate material (125–250mm) and small particles (
ISSN:0378-5173
1873-3476
DOI:10.1016/j.ijpharm.2015.04.020