A genomic region from Pseudomonas fluorescens Pf-5 required for pyrrolnitrin production and inhibition of Pyrenophora tritici-repentis in wheat straw

Pseudomonas fluorescens strain Pf-5, which produces many antifungal metabolites including pyrrolnitrin, inhibited mycelial growth of Pyrenophora tritici-repentis in agar culture and suppressed ascocarp formation by the fungus on axenically infested wheat strew kept moist during incubation. Purified...

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Bibliographic Details
Published in:Phytopathology 1993, Vol.83 (11), p.1223-1228
Main Authors: PFENDER, W. F, KRAUS, J, LOPER, J. E
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Biological control
Biotechnology
Control
Fundamental and applied biological sciences. Psychology
Fungal plant pathogens
Phytopathology. Animal pests. Plant and forest protection
Pseudomonas fluorescens
Pyrenophora tritici-repentis
Triticum aestivum
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Summary:Pseudomonas fluorescens strain Pf-5, which produces many antifungal metabolites including pyrrolnitrin, inhibited mycelial growth of Pyrenophora tritici-repentis in agar culture and suppressed ascocarp formation by the fungus on axenically infested wheat strew kept moist during incubation. Purified pyrrolnitrin was similarly inhibitory. A Tn5 mutant of Pf-5 that did not produce detectable levels of pyrrolnitrin did not inhibit P. tritici-repentis on a culture medium or on wheat straw. A genomic region of Pf-5 was cloned that restored the pyrrolnitrin-deficient Tn5 mutant to pyrrolnitrin production and antagonism to P. tritici-repentis in culture. Pf-5 did not persist or suppress P. tritici-repentis on naturally infested wheat straw incubated under simulated field conditions of fluctuating moist and dry periods.
ISSN:0031-949X
1943-7684
DOI:10.1094/Phyto-83-1223