Prevalence of porcine circovirus-2 DNA-positive ovarian and uterine tissues in gilts culled due to reproductive disturbance in Thailand

• Published in: Tropical animal health and production 2015-06, Vol.47 (5), p.833-840
• Main Authors: Pearodwong, Pachara, Srisuwatanasagul, Sayamon, Teankum, Komkrich, Tantilertcharoen, Rachod, Tummaruk, Padet
• Format: Article
• Language: English
• Subjects: Abortion, Veterinary; Animals; Biomedical and Life Sciences; Circoviridae Infections - epidemiology; Circoviridae Infections - veterinary; Circovirus - genetics; Circovirus - isolation & purification; Deoxyribonucleic acid; DNA; DNA, Viral - analysis; Enzyme-Linked Immunosorbent Assay; Female; Life Sciences; Lymphocytes; Ovary - virology; Polymerase Chain Reaction - veterinary; Pregnancy; Prevalence; Regular Articles; Reproduction; Swine; Swine Diseases - epidemiology; Swine Diseases - virology; Thailand - epidemiology; Tissues; Uterus - virology; Veterinary Medicine/Veterinary Science; Zoology
• ISSN: 0049-4747; 1573-7438
• DOI: 10.1007/s11250-015-0796-5

The present study aimed to determine the prevalence of porcine circovirus-2 (PCV-2) DNA-positive ovarian and uterine tissues in gilts culled due to reproductive disturbance in Thailand. Tissues (70 ovaries and 102 uteri) and serum ( n  = 102) samples from 102 gilts were included. PCV-2 DNA was detected by using polymerase chain reactions. The localisation of PCV-2 antigen was determined by immunohistochemistry, and PCV-2 antibody was evaluated by ELISA. PCV-2 DNA was detected in 30.0 % (21/70) of the ovaries and in 45.1 % (46/102) of the uteri. Age did not influence the frequency of PCV-2 DNA detection in these reproductive organs of gilts ( P  > 0.05). The prevalence of PCV-2 DNA-positive uterine tissue in gilts culled due to non-reproductive problems (20.0 %) was lower than gilts culled due to abortion (85.0 %), abnormal vaginal discharge (47.5 %) and anoestrus (53.5 %) ( P