Prevalence of bovine theileriosis in North Central region of Algeria by real-time polymerase chain reaction with a note on its distribution

To determine the presence and distribution of bovine theileriosis in the North Central region of Algeria, 358 DNA samples and 359 blood smears were analyzed from nine provinces. Theileria DNA extracted from cattle blood was amplified by fluorescence resonance energy transfer polymerase chain reactio...

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Bibliographic Details
Published in:Tropical animal health and production 2015-06, Vol.47 (5), p.787-796
Main Authors: Ziam, Hocine, Kelanamer, Rabah, Aissi, Miriem, Ababou, Assia, Berkvens, Dirk, Geysen, Dirk
Format: Article
Language:English
Subjects:
Agricultural land
Algeria - epidemiology
Animals
Base Sequence
Bioclimatology
Biomedical and Life Sciences
Blood
Cattle
Cloning
Demography
Deoxyribonucleic acid
DNA
DNA Primers
DNA, Protozoan - analysis
Energy transfer
Land use
Life Sciences
Molecular Sequence Data
Phylogeny
Prevalence
Real-Time Polymerase Chain Reaction - veterinary
Regular Articles
Theileria annulata - genetics
Theileriasis - blood
Theileriasis - epidemiology
Theileriasis - parasitology
Ticks - parasitology
Veterinary Medicine/Veterinary Science
Zoology
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Summary:To determine the presence and distribution of bovine theileriosis in the North Central region of Algeria, 358 DNA samples and 359 blood smears were analyzed from nine provinces. Theileria DNA extracted from cattle blood was amplified by fluorescence resonance energy transfer polymerase chain reaction (FRET-PCR). Blood smears were examined for Theileria piroplasms by microscopical examination (ME) of Giemsa-stained slides. While microscopical identification revealed only 42 animals being infected with Theileria piroplasms, PCR-positive amplification using Theileria genus-specific primers was obtained from 132 Theileria spp. ( P  
ISSN:0049-4747
1573-7438
DOI:10.1007/s11250-015-0772-0