Lysobacter fragariae sp. nov. and Lysobacter rhizosphaerae sp. nov. isolated from rhizosphere of strawberry plant

• Published in: Antonie van Leeuwenhoek 2015-06, Vol.107 (6), p.1437-1444
• Main Authors: Singh, Hina, Du, Juan, Ngo, Hien T T, Won, KyungHwa, Yang, Jung-Eun, Kim, Ki-Young, Yi, Tae-Hoo
• Format: Article
• Language: English
• Subjects: Bacterial Typing Techniques; Base Composition; Cluster Analysis; Cytosol - chemistry; Deoxyribonucleic acid; DNA; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fatty Acids - analysis; Fragaria - microbiology; Lipids; Lysobacter - classification; Lysobacter - genetics; Lysobacter - isolation & purification; Lysobacter - physiology; Molecular Sequence Data; Nucleic Acid Hybridization; Phospholipids - analysis; Phylogeny; Pigments, Biological - metabolism; Plant Roots - microbiology; Quinones - analysis; Republic of Korea; Rhizosphere; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-015-0439-x

Two bacterial strains, designated THG-DN8.7(T) and THG-DN8.3(T), were isolated from the rhizosphere of a strawberry plant in Gyeryong Mountain, South Korea. Cells of both isolates were observed to be Gram-negative, yellow-coloured and rod-shaped. Comparative 16S rRNA gene sequence analysis showed that strain THG-DN8.7(T) had highest sequence similarities to Lysobacter yangpyeongensis KACC 11407(T) (97.2 %), Lysobacter niabensis KACC 11587(T) (97.0 %) and Lysobacter oryzae KCTC 22249(T) (96.9 %), while strain THG-DN8.3(T) had closely similarity with L. niabensis KACC 11587(T) (98.1 %), L. oryzae KCTC 22249(T) (97.1 %) and L. yangpyeongensis KACC 11407(T) (96.1 %). DNA-DNA relatedness values between strains THG-DN8.7(T) and THG-DN8.3(T) and their closest phylogenetically neighbours were below 30.0 %, which indicates that strains THG-DN8.7(T) and THG-DN8.3(T) represent distinct species within the genus Lysobacter. Both strains were found to contain iso-C15:0, iso-C16:0 and iso-C17:1 ω9c as predominant fatty acids and ubiquinone-8 as major isoprenoid quinone. The major polar lipids were identified as phosphatidylethanolamine, phosphatidyl-N-methylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content of strains THG-DN8.7(T) and THG-DN8.3(T) were determined to be 66.9 and 67.8 mol%, respectively. These data are consistent with the affiliation of the two new species represented by THG-DN8.7(T) and THG-DN8.3(T) to the genus Lysobacter. The names Lysobacter fragariae sp. nov. and Lysobacter rhizosphaerae sp. nov. are proposed for these species with the type strains THG-DN8.7(T) (=KCTC 42236(T) = JCM 30322(T)) and THG-DN8.3(T) (=KCTC 42237(T) = JCM 30321(T)), respectively.