Characterization of onion lectin (Allium cepa agglutinin) as an immunomodulatory protein inducing Th1-type immune response in vitro

• Published in: International immunopharmacology 2015-06, Vol.26 (2), p.304-313
• Main Authors: Prasanna, Vaddi K., Venkatesh, Yeldur P.
• Format: Article
• Language: English
• Subjects: Animals; Cell Line; Cell Proliferation - drug effects; Cytokines - metabolism; Hemagglutination; Immunity, Cellular - drug effects; Immunomodulation; In Vitro Techniques; Inflammation Mediators - metabolism; Lymphoproliferation; Macrophage activation; Macrophages - drug effects; Macrophages - immunology; Mice; Nitric Oxide - metabolism; Onion lectin; Onions - immunology; Plant Lectins - administration & dosage; Plant Lectins - immunology; Plant Lectins - isolation & purification; Rats; Rats, Wistar; Th1 Cells - drug effects; Th1 Cells - immunology; Th1-Th2 Balance; Th1-type immune response
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2015.04.009

Onion (Allium cepa), a bulb crop of economic importance, is known to have many health benefits. The major objective of the present study is to address the immunomodulatory properties of onion lectin (A. cepa agglutinin; ACA). ACA was purified from onion extract by d-mannose-agarose chromatography (yield: ~1mg/kg). ACA is non-glycosylated and showed a molecular mass of ~12kDa under reducing/non-reducing SDS-PAGE; glutaraldehyde cross-linking indicated that ACA is a non-covalent tetramer of ~12kDa subunits. Its N-terminal sequence (RNVLLNNEGL; UniProt KB Accn. C0HJM8) showed 70–90% homology to mannose-specific Allium agglutinins. ACA showed specific hemagglutination activity of 8200units/mg and is stable in the pH range 6–10 and up to 45° C. The immunomodulatory activity of ACA was assessed using the macrophage cell line, RAW264.7 and rat peritoneal macrophages; at 0.1μg/well, it showed a significant increase (6–8-fold vs. control) in the production of nitric oxide at 24h, and significantly stimulated (2–4-fold vs. control) the production of pro-inflammatory cytokines (TNF-α and IL-12) at 24h. ACA (0.1μg/well) enhanced the proliferation of murine thymocytes by ~4 fold (vs. control) at 24h; however, ACA does not proliferate B cell-enriched rat splenocytes. Further, it significantly elevated the expression levels of cytokines (IFN-γ and IL-2) over the control in murine thymocytes. Taken together, purified ACA induces a Th1-type immune response in vitro. Though present in low amounts, ACA may contribute to the immune-boosting potential of the popular spice onion since considerable amounts are consumed on a daily basis universally. [Display omitted] •Purified Allium cepa agglutinin (ACA) is a non-covalent tetramer of 12kDa subunits.•N-terminal sequence (RNVLLNNEGL) of ACA shows 70–90% homology to Allium agglutinins.•ACA induces proliferation of murine thymocytes by ~4-fold compared to control.•ACA shows 6–8-fold increase in NO release from RAW264.7 cells/peritoneal macrophages.•ACA induces Th1-type immune response in vitro based on the cytokine release pattern.