Differences in the hybridization pattern of Bacillus subtilis genes coding for rRNA depend on the method of DNA preparation

Three different methods of DNA isolation have been used to prepare DNA from Bacillus subtilis. Subsequent hybridization with an rDNA probe produces different patterns, which mirror those previously reported to indicate an rDNA deletion.

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Bibliographic Details
Published in:Applied and environmental microbiology 1993, Vol.59 (3), p.919-921
Main Authors: WATERHOUSE, R. N, GLOVER, L. A
Format: Article
Language:English
Subjects:
Bacillus subtilis
Bacteria
Biological and medical sciences
Cellular biology
Deoxyribonucleic acid
Diverse techniques
DNA
Fundamental and applied biological sciences. Psychology
Genetics
Molecular and cellular biology
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Description
Summary:Three different methods of DNA isolation have been used to prepare DNA from Bacillus subtilis. Subsequent hybridization with an rDNA probe produces different patterns, which mirror those previously reported to indicate an rDNA deletion.
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.59.3.919-921.1993