Immunomodulatory effect of sodium dodecyl sulfate on human neutrophils and the human promyelocytic HL-60 cell line

• Published in: European journal of pharmacology 1994-08, Vol.270 (4), p.285-289
• Main Authors: Kacani, Ladislav, Ferencik, Miroslav, Devinsky, Ferdinand, Möst, Johannes, Dierich, Manfred P.
• Format: Article
• Language: English
• Subjects: Adjuvants, Immunologic - pharmacology; Biological and medical sciences; Cell Division - drug effects; Detergent; Glucuronidase - metabolism; HL-60 cell line; Humans; Immunomodulators; Immunotoxicology; Leukemia, Myeloid - immunology; Lysosomes - drug effects; Lysosomes - enzymology; Medical sciences; Neutrophil, human; Neutrophils - drug effects; Neutrophils - immunology; Peroxidase - metabolism; Pharmacology. Drug treatments; Respiratory burst; Respiratory Burst - drug effects; Sodium dodecyl sulfate (SDS); Sodium Dodecyl Sulfate - pharmacology; Superoxides - metabolism; Tetradecanoylphorbol Acetate - pharmacology; Thymidine - metabolism; Tumor Cells, Cultured
• ISSN: 0926-6917; 0014-2999
• DOI: 10.1016/0926-6917(94)90003-5

The effect of sodium dodecyl sulfate (SDS), an anionic amphiphilic detergent, on the function of human neutrophils and of the human promyelocytic leukemia cell line HL-60 was investigated. SDS modulated the respiratory burst in human neutrophils and HL-60 cells which were stimulated with phorbol 12-myristate 13-acetate (PMA). In concentrations above 1 × 10 −6 M it also caused release of lysosomal enzymes (β- d-glucuronidase, myeloperoxidase and lysozyme) from neutrophils. Our results demonstrate that SDS at concentrations 1 × 10 −6 M-1 × 10 −4 M strongly affect properties of human phagocytic cells.