Characterization of the Bioactive Metabolites from a Plant Growth-Promoting Rhizobacteria and Their Exploitation as Antimicrobial and Plant Growth-Promoting Agents

• Published in: Applied biochemistry and biotechnology 2015-05, Vol.176 (2), p.529-546
• Main Authors: George, Emrin, Kumar, S. Nishanth, Jacob, Jubi, Bommasani, Bhaskara, Lankalapalli, Ravi S, Morang, P, Kumar, B. S. Dileep
• Format: Article
• Language: English
• Subjects: agar; antibacterial properties; Antifungal Agents - chemistry; Antifungal Agents - isolation & purification; Antifungal Agents - metabolism; antifungal properties; Antimicrobial agents; aseptic conditions; Aspergillus flavus; Aspergillus fumigatus; Aspergillus niger; Aspergillus tubingensis; Bacteria; Biochemistry; Biotechnology; Cajanus cajan; Candida albicans; Chemistry; Chemistry and Materials Science; Chloroform; cowpeas; crops; field experimentation; Fungi; Fungi - growth & development; Fusarium oxysporum; genes; Glomerella cingulata; growth promotion; humans; Metabolites; Pathogens; pigeon peas; Plant extracts; Plant growth; plant growth-promoting rhizobacteria; Plant Roots - microbiology; Pseudomonas aeruginosa; Pseudomonas aeruginosa - metabolism; pyocyanin; ribosomal DNA; soil; Soil Microbiology; solvents; spectral analysis; tea; Thanatephorus cucumeris; Trichophyton rubrum; Vigna unguiculata
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-015-1593-3

A plant growth-promoting bacterial strain, PM 105, isolated from a tea plantation soil from the North Eastern region of India was identified as Pseudomonas aeruginosa through classical and 16S ribosomal DNA (rDNA) gene sequencing. Further studies with this strain confirmed broad spectrum antifungal activity against ten human and plant pathogenic fungal pathogens viz. Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Aspergillus tubingensis, Candida albicans, Colletotrichum gloeosporioides, Fusarium oxysporum, Pencillium expansum, Rhizoctonia solani, Trichophyton rubrum besides growth-promoting property in cowpea (Vigna unguiculata) and pigeon pea (Cajanus cajan). However, no antibacterial property was exhibited by this strain against the four test bacterial pathogens tested in agar overlay method. The crude bioactive metabolites produced by this strain were isolated with three different solvents that exhibited significant antimicrobial and plant growth-promoting activity. Chloroform extract recorded significant antimicrobial and plant growth-promoting activity. Three major compounds viz. 1-hydroxyphenazine, pyocyanin, and phenazine-1-carboxamide were purified and characterized from crude extracts of this strain by various spectral data. The purified compounds recorded prominent antimicrobial activity but failed to establish the plant growth promotion activity in test crop plants under gnotobiotic conditions. Pyocyanin recorded significant antimicrobial activity, and best activity was recorded against T. rubrum (29 mm), followed by P. expansum (28 mm). These results suggest the use of PM 105 as plant growth-promoting agent in crop plants after successful field trials.