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Immunogenicity and anti-fecundity effect of nanoparticle coated glutathione S-transferase ( Sj GST) DNA vaccine against murine Schistosoma japonicum infection
Abstract There is still urgent need for a vaccine against schistosomiasis, especially in Schistosoma japonicum endemic areas where even a vaccine that will interrupt zoonotic transmission will be potentially effective as an intervention tool. We had developed a novel nanoparticle gene delivery syste...
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Published in: | Parasitology international 2015-08, Vol.64 (4), p.24-31 |
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creator | Mbanefo, Evaristus Chibunna Kumagai, Takashi Kodama, Yukinobu Kurosaki, Tomoaki Furushima-Shimogawara, Rieko Cherif, Mahamoud Sama Mizukami, Shusaku Kikuchi, Mihoko Huy, Nguyen Tien Ohta, Nobuo Sasaki, Hitoshi Hirayama, Kenji |
description | Abstract There is still urgent need for a vaccine against schistosomiasis, especially in Schistosoma japonicum endemic areas where even a vaccine that will interrupt zoonotic transmission will be potentially effective as an intervention tool. We had developed a novel nanoparticle gene delivery system, which has proven efficacious in gene transfection to target immune cells with complementary adjuvant effect and high protective efficacy in several diseases. Here, we applied this nanoparticle system in combination with S. japonicum glutathione S-transferase ( Sj GST) DNA vaccine to show the immunogenicity and anti-fecundity effect of the nanoparticle coated vaccine formulation against murine schistosomiasis. The nanoparticle-coated DNA vaccine formulation induced desired immune responses. In comparison with the nanoparticle coated empty vector, it produced significantly increased antigen-specific humoral response, T-helper 1 polarized cytokine environment, higher proportion of IFN-γ producing CD4+ T-cells and the concomitant decrease in IL-4 producing CD4+ T-cells. Although there was no effect on worm burden, we recorded a marked reduction in tissue egg burden. There was up to 71.3% decrease in tissue egg burden and 55% reduction in the fecundity of female adult worms. Our data showed that Sj GST DNA vaccine, delivered using the nanoparticle gene delivery system, produced anti-fecundity effect on female adult schistosomes as previously described by using conventional subunit vaccine with adjuvant, proving this DNA vaccine formulation as a promising candidate for anti-pathology and transmission blocking application. |
doi_str_mv | 10.1016/j.parint.2015.01.005 |
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We had developed a novel nanoparticle gene delivery system, which has proven efficacious in gene transfection to target immune cells with complementary adjuvant effect and high protective efficacy in several diseases. Here, we applied this nanoparticle system in combination with S. japonicum glutathione S-transferase ( Sj GST) DNA vaccine to show the immunogenicity and anti-fecundity effect of the nanoparticle coated vaccine formulation against murine schistosomiasis. The nanoparticle-coated DNA vaccine formulation induced desired immune responses. In comparison with the nanoparticle coated empty vector, it produced significantly increased antigen-specific humoral response, T-helper 1 polarized cytokine environment, higher proportion of IFN-γ producing CD4+ T-cells and the concomitant decrease in IL-4 producing CD4+ T-cells. Although there was no effect on worm burden, we recorded a marked reduction in tissue egg burden. There was up to 71.3% decrease in tissue egg burden and 55% reduction in the fecundity of female adult worms. Our data showed that Sj GST DNA vaccine, delivered using the nanoparticle gene delivery system, produced anti-fecundity effect on female adult schistosomes as previously described by using conventional subunit vaccine with adjuvant, proving this DNA vaccine formulation as a promising candidate for anti-pathology and transmission blocking application.</description><identifier>ISSN: 1383-5769</identifier><identifier>EISSN: 1873-0329</identifier><identifier>DOI: 10.1016/j.parint.2015.01.005</identifier><identifier>PMID: 25603531</identifier><language>eng</language><publisher>Netherlands: Elsevier Ireland Ltd</publisher><subject>Animals ; Anti-fecundity ; CD4-Positive T-Lymphocytes - immunology ; Cytokines - immunology ; DNA vaccine ; Female ; Fertility ; Gastroenterology and Hepatology ; Gene delivery ; Glutathione S-transferase (GST) ; Glutathione Transferase - genetics ; Immunity, Cellular ; Immunity, Humoral ; Infectious Disease ; Interferon-gamma - immunology ; Interleukin-4 - immunology ; Mice ; Nanoparticle ; Nanoparticles ; Schistosoma japonicum ; Schistosoma japonicum - immunology ; Schistosoma japonicum - physiology ; Schistosomiasis japonica - parasitology ; Schistosomiasis japonica - prevention & control ; Transfection ; Vaccination - methods ; Vaccines, DNA - administration & dosage ; Vaccines, DNA - immunology</subject><ispartof>Parasitology international, 2015-08, Vol.64 (4), p.24-31</ispartof><rights>Elsevier Ireland Ltd</rights><rights>2015 Elsevier Ireland Ltd</rights><rights>Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-c4798d11ed30b01e63b6c50a10a80af1a066516d841d84bb9cbbcbfb343cfda03</citedby><cites>FETCH-LOGICAL-c441t-c4798d11ed30b01e63b6c50a10a80af1a066516d841d84bb9cbbcbfb343cfda03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25603531$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mbanefo, Evaristus Chibunna</creatorcontrib><creatorcontrib>Kumagai, Takashi</creatorcontrib><creatorcontrib>Kodama, Yukinobu</creatorcontrib><creatorcontrib>Kurosaki, Tomoaki</creatorcontrib><creatorcontrib>Furushima-Shimogawara, Rieko</creatorcontrib><creatorcontrib>Cherif, Mahamoud Sama</creatorcontrib><creatorcontrib>Mizukami, Shusaku</creatorcontrib><creatorcontrib>Kikuchi, Mihoko</creatorcontrib><creatorcontrib>Huy, Nguyen Tien</creatorcontrib><creatorcontrib>Ohta, Nobuo</creatorcontrib><creatorcontrib>Sasaki, Hitoshi</creatorcontrib><creatorcontrib>Hirayama, Kenji</creatorcontrib><title>Immunogenicity and anti-fecundity effect of nanoparticle coated glutathione S-transferase ( Sj GST) DNA vaccine against murine Schistosoma japonicum infection</title><title>Parasitology international</title><addtitle>Parasitol Int</addtitle><description>Abstract There is still urgent need for a vaccine against schistosomiasis, especially in Schistosoma japonicum endemic areas where even a vaccine that will interrupt zoonotic transmission will be potentially effective as an intervention tool. We had developed a novel nanoparticle gene delivery system, which has proven efficacious in gene transfection to target immune cells with complementary adjuvant effect and high protective efficacy in several diseases. Here, we applied this nanoparticle system in combination with S. japonicum glutathione S-transferase ( Sj GST) DNA vaccine to show the immunogenicity and anti-fecundity effect of the nanoparticle coated vaccine formulation against murine schistosomiasis. The nanoparticle-coated DNA vaccine formulation induced desired immune responses. In comparison with the nanoparticle coated empty vector, it produced significantly increased antigen-specific humoral response, T-helper 1 polarized cytokine environment, higher proportion of IFN-γ producing CD4+ T-cells and the concomitant decrease in IL-4 producing CD4+ T-cells. Although there was no effect on worm burden, we recorded a marked reduction in tissue egg burden. There was up to 71.3% decrease in tissue egg burden and 55% reduction in the fecundity of female adult worms. Our data showed that Sj GST DNA vaccine, delivered using the nanoparticle gene delivery system, produced anti-fecundity effect on female adult schistosomes as previously described by using conventional subunit vaccine with adjuvant, proving this DNA vaccine formulation as a promising candidate for anti-pathology and transmission blocking application.</description><subject>Animals</subject><subject>Anti-fecundity</subject><subject>CD4-Positive T-Lymphocytes - immunology</subject><subject>Cytokines - immunology</subject><subject>DNA vaccine</subject><subject>Female</subject><subject>Fertility</subject><subject>Gastroenterology and Hepatology</subject><subject>Gene delivery</subject><subject>Glutathione S-transferase (GST)</subject><subject>Glutathione Transferase - genetics</subject><subject>Immunity, Cellular</subject><subject>Immunity, Humoral</subject><subject>Infectious Disease</subject><subject>Interferon-gamma - immunology</subject><subject>Interleukin-4 - immunology</subject><subject>Mice</subject><subject>Nanoparticle</subject><subject>Nanoparticles</subject><subject>Schistosoma japonicum</subject><subject>Schistosoma japonicum - immunology</subject><subject>Schistosoma japonicum - physiology</subject><subject>Schistosomiasis japonica - parasitology</subject><subject>Schistosomiasis japonica - prevention & control</subject><subject>Transfection</subject><subject>Vaccination - methods</subject><subject>Vaccines, DNA - administration & dosage</subject><subject>Vaccines, DNA - immunology</subject><issn>1383-5769</issn><issn>1873-0329</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqFUk1v1DAQjRCIlsI_QMjHcsgys9587AWpammpVMEh5WxNnMnWIbEX26m0f4bfWkdbOHDB0njG1pt59rzJsvcIKwQsPw2rPXlj42oNWKwAVwDFi-wU60rmINfblymWtcyLqtyeZG9CGCABqwpfZyfrogRZSDzNft9O02zdjq3RJh4E2S5ZNHnPerbdcsV9iqNwvbBkXSKNRo8stKPIndiNc6T4YJxl0eTRkw09ewoszkUziJvm_qO4-nYhHklrkzC0I2NDFNPsl2OjH0yILriJxEB7l54xT8LYhTLVfJu96mkM_O7Zn2U_rr_cX37N777f3F5e3OV6s8GY9mpbd4jcSWgBuZRtqQsgBKqBeiQoywLLrt5gsrbd6rbVbd_KjdR9RyDPsvNj3b13v2YOUU0maB5HsuzmoLCsJVRFtZYJujlCtXcheO7V3puJ_EEhqEUZNaijMmpRRgGqpExK-_DMMLcTd3-T_kiRAJ-PAE7_fDTsVdCGrebO-NQM1TnzP4Z_C-jRpH7S-JMPHAY3e5t6qFCFtQLVLNOxDAcWkFa5lU-5NLlv</recordid><startdate>20150801</startdate><enddate>20150801</enddate><creator>Mbanefo, Evaristus Chibunna</creator><creator>Kumagai, Takashi</creator><creator>Kodama, Yukinobu</creator><creator>Kurosaki, Tomoaki</creator><creator>Furushima-Shimogawara, Rieko</creator><creator>Cherif, Mahamoud Sama</creator><creator>Mizukami, Shusaku</creator><creator>Kikuchi, Mihoko</creator><creator>Huy, Nguyen Tien</creator><creator>Ohta, Nobuo</creator><creator>Sasaki, Hitoshi</creator><creator>Hirayama, Kenji</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150801</creationdate><title>Immunogenicity and anti-fecundity effect of nanoparticle coated glutathione S-transferase ( Sj GST) DNA vaccine against murine Schistosoma japonicum infection</title><author>Mbanefo, Evaristus Chibunna ; Kumagai, Takashi ; Kodama, Yukinobu ; Kurosaki, Tomoaki ; Furushima-Shimogawara, Rieko ; Cherif, Mahamoud Sama ; Mizukami, Shusaku ; Kikuchi, Mihoko ; Huy, Nguyen Tien ; Ohta, Nobuo ; Sasaki, Hitoshi ; Hirayama, Kenji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-c4798d11ed30b01e63b6c50a10a80af1a066516d841d84bb9cbbcbfb343cfda03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Anti-fecundity</topic><topic>CD4-Positive T-Lymphocytes - immunology</topic><topic>Cytokines - immunology</topic><topic>DNA vaccine</topic><topic>Female</topic><topic>Fertility</topic><topic>Gastroenterology and Hepatology</topic><topic>Gene delivery</topic><topic>Glutathione S-transferase (GST)</topic><topic>Glutathione Transferase - genetics</topic><topic>Immunity, Cellular</topic><topic>Immunity, Humoral</topic><topic>Infectious Disease</topic><topic>Interferon-gamma - immunology</topic><topic>Interleukin-4 - immunology</topic><topic>Mice</topic><topic>Nanoparticle</topic><topic>Nanoparticles</topic><topic>Schistosoma japonicum</topic><topic>Schistosoma japonicum - immunology</topic><topic>Schistosoma japonicum - physiology</topic><topic>Schistosomiasis japonica - parasitology</topic><topic>Schistosomiasis japonica - prevention & control</topic><topic>Transfection</topic><topic>Vaccination - methods</topic><topic>Vaccines, DNA - administration & dosage</topic><topic>Vaccines, DNA - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mbanefo, Evaristus Chibunna</creatorcontrib><creatorcontrib>Kumagai, Takashi</creatorcontrib><creatorcontrib>Kodama, Yukinobu</creatorcontrib><creatorcontrib>Kurosaki, Tomoaki</creatorcontrib><creatorcontrib>Furushima-Shimogawara, Rieko</creatorcontrib><creatorcontrib>Cherif, Mahamoud Sama</creatorcontrib><creatorcontrib>Mizukami, Shusaku</creatorcontrib><creatorcontrib>Kikuchi, Mihoko</creatorcontrib><creatorcontrib>Huy, Nguyen Tien</creatorcontrib><creatorcontrib>Ohta, Nobuo</creatorcontrib><creatorcontrib>Sasaki, Hitoshi</creatorcontrib><creatorcontrib>Hirayama, Kenji</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mbanefo, Evaristus Chibunna</au><au>Kumagai, Takashi</au><au>Kodama, Yukinobu</au><au>Kurosaki, Tomoaki</au><au>Furushima-Shimogawara, Rieko</au><au>Cherif, Mahamoud Sama</au><au>Mizukami, Shusaku</au><au>Kikuchi, Mihoko</au><au>Huy, Nguyen Tien</au><au>Ohta, Nobuo</au><au>Sasaki, Hitoshi</au><au>Hirayama, Kenji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunogenicity and anti-fecundity effect of nanoparticle coated glutathione S-transferase ( Sj GST) DNA vaccine against murine Schistosoma japonicum infection</atitle><jtitle>Parasitology international</jtitle><addtitle>Parasitol Int</addtitle><date>2015-08-01</date><risdate>2015</risdate><volume>64</volume><issue>4</issue><spage>24</spage><epage>31</epage><pages>24-31</pages><issn>1383-5769</issn><eissn>1873-0329</eissn><abstract>Abstract There is still urgent need for a vaccine against schistosomiasis, especially in Schistosoma japonicum endemic areas where even a vaccine that will interrupt zoonotic transmission will be potentially effective as an intervention tool. We had developed a novel nanoparticle gene delivery system, which has proven efficacious in gene transfection to target immune cells with complementary adjuvant effect and high protective efficacy in several diseases. Here, we applied this nanoparticle system in combination with S. japonicum glutathione S-transferase ( Sj GST) DNA vaccine to show the immunogenicity and anti-fecundity effect of the nanoparticle coated vaccine formulation against murine schistosomiasis. The nanoparticle-coated DNA vaccine formulation induced desired immune responses. In comparison with the nanoparticle coated empty vector, it produced significantly increased antigen-specific humoral response, T-helper 1 polarized cytokine environment, higher proportion of IFN-γ producing CD4+ T-cells and the concomitant decrease in IL-4 producing CD4+ T-cells. Although there was no effect on worm burden, we recorded a marked reduction in tissue egg burden. There was up to 71.3% decrease in tissue egg burden and 55% reduction in the fecundity of female adult worms. Our data showed that Sj GST DNA vaccine, delivered using the nanoparticle gene delivery system, produced anti-fecundity effect on female adult schistosomes as previously described by using conventional subunit vaccine with adjuvant, proving this DNA vaccine formulation as a promising candidate for anti-pathology and transmission blocking application.</abstract><cop>Netherlands</cop><pub>Elsevier Ireland Ltd</pub><pmid>25603531</pmid><doi>10.1016/j.parint.2015.01.005</doi><tpages>8</tpages></addata></record> |
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subjects | Animals Anti-fecundity CD4-Positive T-Lymphocytes - immunology Cytokines - immunology DNA vaccine Female Fertility Gastroenterology and Hepatology Gene delivery Glutathione S-transferase (GST) Glutathione Transferase - genetics Immunity, Cellular Immunity, Humoral Infectious Disease Interferon-gamma - immunology Interleukin-4 - immunology Mice Nanoparticle Nanoparticles Schistosoma japonicum Schistosoma japonicum - immunology Schistosoma japonicum - physiology Schistosomiasis japonica - parasitology Schistosomiasis japonica - prevention & control Transfection Vaccination - methods Vaccines, DNA - administration & dosage Vaccines, DNA - immunology |
title | Immunogenicity and anti-fecundity effect of nanoparticle coated glutathione S-transferase ( Sj GST) DNA vaccine against murine Schistosoma japonicum infection |
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