Supercritical fluid extraction and HPLC analysis of benzimidazole fungicides in potato, apple and banana

An analytical method employing supercritical fluid extraction (SFE) of the benzimidazole fungicides thiabendazole, benomyl/carbendazim, and thiophanate-methyl was developed and tested with potato, banana, and apple matrices. Ion-pairing HPLC coupled with UV absorbance or fluorescence detection was u...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1994-12, Vol.42 (12), p.2817-2823
Main Authors: Aharonson, Nadav, Lehotay, Steven J, Ibrahim, Medina A
Format: Article
Language:English
Subjects:
BANANE
BANANO
BENZIMIDAZOLE
BENZIMIDAZOLES
Biological and medical sciences
CHROMATOGRAPHIE LIQUIDE HAUTE PRESS
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
CROMATOGRAFIA LIQUIDA ALTA PRESION
FONGICIDE
Food industries
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
FUNGICIDAS
MANZANA
PAPA
POMME
POMME DE TERRE
RESIDU
RESIDUOS
TECHNIQUE ANALYTIQUE
TECNICAS ANALITICAS
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Summary:An analytical method employing supercritical fluid extraction (SFE) of the benzimidazole fungicides thiabendazole, benomyl/carbendazim, and thiophanate-methyl was developed and tested with potato, banana, and apple matrices. Ion-pairing HPLC coupled with UV absorbance or fluorescence detection was used for analysis. Sample preparation involved shredding a 100 g sample, mixing a 3 g subsample with 2 g of Hydromatrix, and packing a 10 mL vessel. Optimal SFE conditions were determined by varying the amount, density, and flow rate of CO2, extraction temperature, type and amount of solvent modifier, and trapping/elution conditions of the extracted pesticides. Optimal conditions for extraction were obtained at 400 atm, 60 degrees C, 90 g of CO2, 3% methanol modifier, and 2 mL/min flow rate. The extracts were collected on a 1 mL C18, trap at 25 degrees C and eluted with 10 mL of methanol. Potato, apple, and banana extracts were analyzed by HPLC without additional cleanup. Recoveries were 90 +/- 12% for the benzimidazole fungicides in fortified samples. Incurred potato and banana samples were also analyzed
ISSN:0021-8561
1520-5118
DOI:10.1021/jf00048a031