DRB1 super()1605: A new DR2-DRB1 allele found in a German family

We are reporting a new DR2 group DRB1 allele found in a German family. This father, mother, and six children were included in different International Histocompatibility Workshops (IHWS) as part of the panel of the Immunogenetics Laboratory in Munich, and so all eight persons had been typed serologic...

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Bibliographic Details
Published in:Immunogenetics (New York) 1994-01, Vol.39 (4), p.300-300
Main Authors: Bettinotti, M P, McNicholas, A, Keller, E, Andreas, A, Scholz, S, Albert, ED
Format: Article
Language:English
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Summary:We are reporting a new DR2 group DRB1 allele found in a German family. This father, mother, and six children were included in different International Histocompatibility Workshops (IHWS) as part of the panel of the Immunogenetics Laboratory in Munich, and so all eight persons had been typed serologically for the HLA class I and II alleles as well as by restriction fragment length polymorphism (RFLP) for the class II alleles. During the 11th IHWS, in which the class II alleles were tested by polymerase chain reaction (PCR) amplification and hybridization with oligonucleotide probes, we found a DRB1 gene reaction pattern for a haplotype shared by the mother and two sons, which did not coincide with previously reported sequences. This led us to sequence the second exon of the DRB1 gene. The new DRB1 allele DRB1 super(*)1605 was typed as DR2 by DRB1 PCR/oligonucleotide probe generic typing, as DR2b by RFLP, and as DR16 by serology.
ISSN:0093-7711