Time lapse microscopy observation of cellular structural changes and image analysis of drug treated cancer cells to characterize the cellular heterogeneity

The effect of Calotropis gigantea latex (CGLX) on human mammary carcinoma cells is not well established. We present the results of this drug activity at total population and single cell level. CGLX inhibited the growth of MCF7 cancer cells at lower IC50 concentration (17 µL/mL). Microscopy of IC50 d...

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Bibliographic Details
Published in:Environmental toxicology 2015-05, Vol.30 (6), p.724-734
Main Authors: Vaiyapuri, Periasamy S, Ali, Alshatwi A, Mohammad, Akbarsha A, Kandhavelu, Jeyalakshmi, Kandhavelu, Meenakshisundaram
Format: Article
Language:English
Subjects:
Antineoplastic Agents, Phytogenic - pharmacology
apoptosis
Apoptosis - drug effects
breast neoplasms
Calotropis
Calotropis - chemistry
Calotropis gigantea
Cell Line, Tumor
cell proliferation
Cell Proliferation - drug effects
complementary and alternative medicine
DNA damage
drugs
Female
growth retardation
heterogeneity
Humans
image analysis
inhibitory concentration 50
latex
mammary carcinoma
Membrane Potential, Mitochondrial - drug effects
microscopy
Microscopy - methods
mitochondria
Mitochondria - drug effects
Mitochondria - metabolism
necrosis
Necrosis - metabolism
Necrosis - pathology
neoplasm cells
Neoplasms - pathology
single cell imaging
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Summary:The effect of Calotropis gigantea latex (CGLX) on human mammary carcinoma cells is not well established. We present the results of this drug activity at total population and single cell level. CGLX inhibited the growth of MCF7 cancer cells at lower IC50 concentration (17 µL/mL). Microscopy of IC50 drug treated cells at 24 hr confirming the appearance of morphological characteristics of apoptotic and necrotic cells, associated with 70% of DNA damage. FACS analysis confirmed that, 10 and 20% of the disruption of cellular mitochondrial nature by at 24 and 48 h, respectively. Microscopic image analysis of total population level proved that MMP changes were statistically significant with P values. The cell to cell variation was confirmed by functional heterogeneity analysis which proves that CGLX was able to induce the apoptosis without the contribution of mitochondria. We conclude that CGLX inhibits cell proliferation, survival, and heterogeneity of pathways in human mammary carcinoma cells. © 2014 Wiley Periodicals, Inc. Environ Toxicol 30: 724–734, 2015.
ISSN:1520-4081
1522-7278
DOI:10.1002/tox.21950