Evaluation of antimicrobial activity of extracts of in vivo and in vitro grown Vinca rosea L. (Catharanthus roseus) against pathogens

The antimicrobial activity of Vinca rosea was evaluated against pathogenic bacterial strains (Bacillus subtilis, B. licheniformis and Azotobacter sp.) and fungal strains (Asprgillus niger, Alternaria solani and Rhizopus oryzae) using agar well diffusion method. Methanolic extracts of in vivo leaf, i...

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Bibliographic Details
Published in:Pakistan journal of pharmaceutical sciences 2015-05, Vol.28 (3), p.849-853
Main Authors: Naz, Shagufta, Haq, Rukhama, Aslam, Farah, Ilyas, Saiqa
Format: Article
Language:English
Subjects:
Alternaria - drug effects
Anti-Infective Agents - pharmacology
Aspergillus niger - drug effects
Azotobacter - drug effects
Bacillus - drug effects
Bacillus subtilis - drug effects
Botanical research
Catharanthus
Fungi - drug effects
Health aspects
In Vitro Techniques
Madagascar periwinkle
Materia medica, Vegetable
Microbial Sensitivity Tests
Pharmacology, Experimental
Phytochemistry
Plant Components, Aerial
Plant extracts
Plant Extracts - pharmacology
Rhizopus - drug effects
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Summary:The antimicrobial activity of Vinca rosea was evaluated against pathogenic bacterial strains (Bacillus subtilis, B. licheniformis and Azotobacter sp.) and fungal strains (Asprgillus niger, Alternaria solani and Rhizopus oryzae) using agar well diffusion method. Methanolic extracts of in vivo leaf, in vitro leaf, in vitro calluses of leaf, nodal and fruit explants were used and exhibited antimicrobial activity as indicated by minimum inhibitory concentration (MIC). In vitro extracts showed better results as compared to the in vivo extracts for both the antibacterial as well as the antifungal activity. Among all the extracts, maximum zone of inhibition (30.3 mm ± 0.58(a)) was formed by in vitro leaf callus extract concentration of 2.0mg/ml against B. licheniformis. Similarly in case of antifungal activity, maximum zone of inhibition (34.6mm ± 0.57(a)) was formed by in vitro leaf callus extract and MIC value is 6.0mg/ml against A. niger. Hence these results clearly depicts that V. rosea possess a great strength to fight against the microbial activity and can be used against various infections.
ISSN:1011-601X