Motility initiation of sterlet sturgeon (Acipenser ruthenus) spermatozoa: Describing the propagation of the first flagellar waves

In the present study, for the first time in fish spermatozoa, we describe the precise chronology of motility initiation of sterlet (sturgeon) sperm from completely immotile flagella to regular full wave propagation. The successive activation steps were investigated by high-speed video microscopy, us...

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Bibliographic Details
Published in:Theriogenology 2015-07, Vol.84 (1), p.51-61
Main Authors: Prokopchuk, Galina, Dzyuba, Boris, Bondarenko, Olga, Rodina, Marek, Cosson, Jacky
Format: Article
Language:English
Subjects:
Acipenser ruthenus
Animals
Aquaculture
DMSO
Fishes - physiology
Flagella - physiology
Flagella movement
High-speed video microscopy
Male
Sperm Motility
Sperm motility initiation
Spermatozoa - cytology
Spermatozoa - physiology
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Summary:In the present study, for the first time in fish spermatozoa, we describe the precise chronology of motility initiation of sterlet (sturgeon) sperm from completely immotile flagella to regular full wave propagation. The successive activation steps were investigated by high-speed video microscopy, using specific experimental situation, where sperm motility initiation was delayed in time up to several seconds (10 ± 2.68 seconds). Starting from fully immotile, the flagellum shows some trembling for a brief period, soon followed by appearance of the first real bend (so-called “principal bend”) with a large wave amplitude 4.28 ± 0.65 μm, then by the “reverse bend,” the latter presenting a lower (P 
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2015.02.011