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Expression of hypoxia-inducible factors and vascular endothelial growth factor during pregnancy in the feline uterus

Hypoxia-inducible factors (HIFs) and vascular endothelial growth factor (VEGF) have critical roles during the development of the fetomaternal unit. The HIFs regulate placentation and vascularization by stimulation of VEGF gene expression. This study aimed to investigate the expression profiles of HI...

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Bibliographic Details
Published in:Theriogenology 2015-07, Vol.84 (1), p.24-33
Main Authors: Agaoglu, Ozgecan Korkmaz, Agaoglu, Ali Reha, Guzeloglu, Aydin, Kurar, Ercan, Kayis, Seyit Ali, Ozmen, Ozlem, Schäfer-Somi, Sabine, Aslan, Selim
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Language:English
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Summary:Hypoxia-inducible factors (HIFs) and vascular endothelial growth factor (VEGF) have critical roles during the development of the fetomaternal unit. The HIFs regulate placentation and vascularization by stimulation of VEGF gene expression. This study aimed to investigate the expression profiles of HIF gene family and VEGF in the cat uterus during pregnancy. Tissue samples of the whole uterine wall were collected after ovariohysterectomy and allocated to the following groups: embryo positive (group 1 [G1], n = 7, 7 days after mating), early pregnancy (group 2 [G2], n = 7, 20 days after mating), mid-pregnancy (group 3 [G3], n = 7, 24 days after mating), late pregnancy (group 4 [G4], n = 7, 30–45 days after mating), and oocyte positive groups (group 5 [G5], n = 7, 7 days after induction of ovulation with GnRH analog). Relative mRNA levels were determined by real-time polymerase chain reaction. As housekeeping gene, glyceraldehyde-3-phosphate dehydrogenase was used. The relative gene expression of HIF1A in G5 was found to be significantly higher than that of other groups (G1, G2, G3, and G4) (P 
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2015.02.009