Diagnostic utility of fractionated urinary filarial antigen

Urinary filarial antigen isolated from urine samples of microfilaraemic patients was analysed for its antigenic activity by immunoblotting and enzyme linked immunosorbent assay techniques. SDS-PAGE fractionation of urinary filarial antigen showed 11 protein bands, of which two showed reactivity with...

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Bibliographic Details
Published in:Journal of biosciences 1993-09, Vol.18 (3), p.319-326
Main Authors: CHENTHAMARAKSHAN, V, PADIGEL, U. M, RAMAPRASAD, P, REDDY, M. V. R, HARINATH, B. C
Format: Article
Language:English
Subjects:
Biological and medical sciences
Diseases caused by nematodes
Filariases
Helminthic diseases
Infectious diseases
Medical sciences
Miscellaneous
Parasitic diseases
Wuchereria bancrofti
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Summary:Urinary filarial antigen isolated from urine samples of microfilaraemic patients was analysed for its antigenic activity by immunoblotting and enzyme linked immunosorbent assay techniques. SDS-PAGE fractionation of urinary filarial antigen showed 11 protein bands, of which two showed reactivity with immunoglobulin-G fraction of filarial serum immunoglobulin in immunoblotting. Antigenic analysis of SDS-PAGE fractions of urinary filarial antigen by inhibition enzyme linked immunosorbent assay using filarial serum immunoglobulin-G and Wuchereria bancrofti microfilarial excretory-secretory antigen revealed 3 fractions, numbers 5, 6 and 9 with significant activity. There was no phosphorylcholine epitope in these fractions. Fractions 5 and 6 can be a candidate antigens for the immunodiagnosis of filariasis.
ISSN:0250-5991
0973-7138
DOI:10.1007/BF02702989