Surface roughness dependent osteoblast and fibroblast response on poly(l-lactide) films and electrospun membranes

Poly(l-lactide) electrospun mats with random and aligned fiber orientation and films have been produced with degrees of crystallinity ranging from 0 up to nearly 50%. The overall surface roughness is practically constant irrespective of the sampling areas (1 × 1 µm to 20 × 20 µm) for degrees of crys...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biomedical materials research. Part A 2015-07, Vol.103 (7), p.2260-2268
Main Authors: Ribeiro, Clarisse, Sencadas, Vitor, Areias, Anabela C., Gama, F. Miguel, Lanceros-Méndez, Senentxu
Format: Article
Language:English
Subjects:
3T3 Cells
Animals
Biotecnologia Médica
Cell Proliferation
cell-material interface
Ciências Médicas
Crystallization
Fibroblasts - cytology
Mice
Osteoblasts - cytology
poly(l-lactic acid)
Polyesters - chemistry
Science & Technology
Surface Properties
tissue and biomedical engineering
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Poly(l-lactide) electrospun mats with random and aligned fiber orientation and films have been produced with degrees of crystallinity ranging from 0 up to nearly 50%. The overall surface roughness is practically constant irrespective of the sampling areas (1 × 1 µm to 20 × 20 µm) for degrees of crystallinity below 30%, increasing for higher degrees of crystallinity for the larger sampling areas. Further, due to fiber confinement, surface roughness variations are smaller in electrospun mats. Samples with 50% of crystallinity show the lowest osteoblast and the highest fibroblast proliferation. Therefore, it is verified that higher roughness promotes lower osteoblast but higher fibroblast proliferation. The overall results indicate the relevant role of the sub-microenvironment variations associated to the microscale roughness in determining the different cell responses. Portuguese Foundation for Science and Technology (FCT); contract grant numbers: C/FIS/UI607/2011, PTDC/CTM-NAN/112574/2009, contract grant numbers: SFRH/BPD/63148/2009, SFRH/BPD/90870/2012, Matepro—Optimizing Materials and Processes, Programa Operacional Regional do Norte, Quadro de Referência Estratégico Nacional” (QREN), “Fundo Europeu de Desenvolvimento Regional” (FEDER); contract grant number: NORTE-07–0124-FEDER-000037.
ISSN:1549-3296
1552-4965
1549-3296
DOI:10.1002/jbm.a.35367