Association of flavin adenine dinucleotide with the Arabidopsis blue light receptor CRY1

The Arabidopsis thaliana HY4 gene encodes CRY1, a 75-kilodalton flavoprotein mediating blue light-dependent regulation of seedling development. CRY1 is demonstrated here to noncovalently bind stoichiometric amounts of flavin adenine dinucleotide (FAD). The redox properties of FAD bound by CRY1 inclu...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1995-01, Vol.269 (522), p.968-970
Main Authors: Lin, Chentao, Robertson, DE, Ahmad, M, Riabekas, A A, Schuman Jorns, M, Dutton, P L, Cashmore, A R
Format: Article
Language:English
Subjects:
Arabidopsis thaliana
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Summary:The Arabidopsis thaliana HY4 gene encodes CRY1, a 75-kilodalton flavoprotein mediating blue light-dependent regulation of seedling development. CRY1 is demonstrated here to noncovalently bind stoichiometric amounts of flavin adenine dinucleotide (FAD). The redox properties of FAD bound by CRY1 include an unexpected stability of the neutral radical flavosemiquinone (FADH times ). The absorption of this flavosemiquinone provide a likely explanation for the additional sensitivity exhibited by CRY1-mediated responses in the green region of the visible spectrum. Despite the sequence homology to microbial DNA photolyases, CRY1 was found to have no detectable photolyase activity.
ISSN:0036-8075