Changes in growth culture FDA activity under changing growth conditions

The FDA hydrolysis capacities and bacterial biomass concentrations (estimated by determination of ATP content) of growth cultures prepared from activated sludge and wastewater, were measured to find out whether the FDA activity would reflect bacterial biomass under different physiological states of...

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Bibliographic Details
Published in:Water research (Oxford) 1992, Vol.26 (9), p.1267-1270
Main Authors: Jørgensen, Per E., Eriksen, Thomas, Jensen, Bjørn K.
Format: Article
Language:English
Subjects:
accumulation of enzymes
activated sludge
activity/biomass ratio
ATP content
Biological and medical sciences
Biotechnology
extracellular enzymes
FDA activity
Fundamental and applied biological sciences. Psychology
growth culture
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
wastewater
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Summary:The FDA hydrolysis capacities and bacterial biomass concentrations (estimated by determination of ATP content) of growth cultures prepared from activated sludge and wastewater, were measured to find out whether the FDA activity would reflect bacterial biomass under different physiological states of the bacteria. The FDA activity/ATP ratio was calculated for different concentrations of autoclaved sludge. A faster decay rate of ATP relative to FDA hydrolysis activity was observed, thus causing changes in the ratio. Furthermore, comparison between values obtained from pure cultures and different soils revealed differences up to two orders of magnitude of the ratio. Based on these results it was concluded that the FDA activity should not be applied for measurements of viable biomass in environments in which different physiological conditions occur.
ISSN:0043-1354
1879-2448
DOI:10.1016/0043-1354(92)90188-A