Potassium ions and changes in bacterial DNA supercoiling under osmotic stress

Escherichia coli transiently increases both the [ATP]/[ADP] ratio and the negative supercoiling of plasmid DNA when it is shifted to high osmolarity. Here we report that a mutant lacking all saturable K+ transport systems increases the negative supercoiling of the plasmid DNA under upshock but canno...

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Bibliographic Details
Published in:FEMS microbiology letters 1992-12, Vol.99 (2‐3), p.159-164
Main Authors: Meury, Jean, Kohiyama, Masamichi
Format: Article
Language:English
Subjects:
Action of physical and chemical agents on bacteria
Bacteriology
Biological and medical sciences
DNA supercoiling
DnaK
Escherichia coli
Fundamental and applied biological sciences. Psychology
Microbiology
Osmotic upshock
Potassium ions
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Summary:Escherichia coli transiently increases both the [ATP]/[ADP] ratio and the negative supercoiling of plasmid DNA when it is shifted to high osmolarity. Here we report that a mutant lacking all saturable K+ transport systems increases the negative supercoiling of the plasmid DNA under upshock but cannot further relax DNA. The mutant dnaK756 behaves like the K+ transport mutant.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1992.tb05559.x