Detection of heat‐stable enterotoxin genes among Australian Vibrio cholerae O1 strains

DNA probes derived from the heat‐stable enterotoxin gene of Vibrio cholerae non‐O1 (stn), and the cholera toxin gene (etc), were used to screen 199 strains of V. cholerae O1, which were isolated within Australia from 1977–1986. 13 environmental strains isolated from the riverine environment in South...

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Bibliographic Details
Published in:FEMS microbiology letters 1995-03, Vol.127 (1‐2), p.111-115
Main Authors: Mallard, Kim E., Desmarchelier, Patricia M.
Format: Article
Language:English
Subjects:
Animals
Australia
Bacteriology
Biological and medical sciences
Disease Reservoirs
DNA probe
Enterotoxins - genetics
Enterotoxins - toxicity
Environmental Microbiology
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Genetics
Heat‐stable enterotoxin
Humans
Mice
Microbiology
NAG‐ST
Polymorphism, Restriction Fragment Length
Vibrio cholerae
Vibrio cholerae - classification
Vibrio cholerae - genetics
Vibrio cholerae - isolation & purification
Virulence - genetics
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Summary:DNA probes derived from the heat‐stable enterotoxin gene of Vibrio cholerae non‐O1 (stn), and the cholera toxin gene (etc), were used to screen 199 strains of V. cholerae O1, which were isolated within Australia from 1977–1986. 13 environmental strains isolated from the riverine environment in Southeast Queensland in 1980 and 1981, hybridized with the stn and ctx DNA probes. The concentrated supernatant of 6 of these strains elicited fluid accumulation in the infant mouse assay both before and after heating at 100 °C for 5 min. Genetic relationships among the 13 stn+ strains were studied by a comparison of the rRNA‐RFLPs (ribotyping) and by Southern blot analysis with a stn gene probe. The results indicate that there is a clonal relationship among the Australian stn+ strains and that there is an environmental reservoir of stn genes among Australian V. cholerae O1 isolates.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1995.tb07458.x