NAP1 acts with Clb2 to perform mitotic functions and to suppress polar bud growth in budding yeast

NAP1 is a 60-kD protein that interacts specifically with mitotic cyclins in budding yeast and frogs. We have examined the ability of the yeast mitotic cyclin Clb2 to function in cells that lack NAP1. Our results demonstrate that Clb2 is unable to carry out its full range of functions without NAP1, e...

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Bibliographic Details
Published in:The Journal of cell biology 1995-08, Vol.130 (3), p.675-685
Main Authors: Kellogg, D.R. (University of California, Santa Cruz, CA.), Murray, A.W
Format: Article
Language:English
Subjects:
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
Animal cells
Cell cycle
Cell division
Cell growth
Cells
Cyclins
DIVISION CELLULAIRE
DIVISION CELULAR
FOSFORILACION
HeLa cells
Microtubules
MITOSE
MITOSIS
Mitotic spindle apparatus
PHOSPHORYLATION
PROTEINA QUINASA
PROTEINAS
PROTEINAS AGLUTINANTES
PROTEINE
PROTEINE DE LIAISON
PROTEINE KINASE
Proteins
SACCHAROMYCES CEREVISIAE
Yeast
Yeasts
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Summary:NAP1 is a 60-kD protein that interacts specifically with mitotic cyclins in budding yeast and frogs. We have examined the ability of the yeast mitotic cyclin Clb2 to function in cells that lack NAP1. Our results demonstrate that Clb2 is unable to carry out its full range of functions without NAP1, even though Clb2 /p34CDC28-associated kinase activity rises to normal levels. In the absence of NAP1, Clb2 is unable to efficiently induce mitotic events, and cells undergo a prolonged delay at the short spindle stage with normal levels of Clb2/p34CDC28 kinase activity. NAP1 is also required for the ability of Clb2 to induce the switch from polar to isotropic bud growth. As a result, polar bud growth continues during mitosis, giving rise to highly elongated cells. Our experiments also suggest that NAP1 is required for the ability of the Clb2/p34CDC28 kinase complex to amplify its own production, and that NAP1 plays a role in regulation of microtubule dynamics during mitosis. Together, these results demonstrate that NAP1 is required for the normal function of the activated Clb2/p34CDC28 kinase complex, and provide a step towards understanding how cyclin-dependent kinase complexes induce specific events during the cell cycle
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.130.3.675