Immunohistochemical analysis of dichloroacetic acid (DCA)-induced hepatocarcinogenesis in male Fischer (F344) rats

We examined the incidence of proliferative lesions, hyperplastic nodules and altered hepatic foci, in male F344 rat liver, to determine their preneoplastic potential during dichloroacetic acid (DCA)-induced hepatocarcinogenesis. Immunohistochemical and image analysis methods were used to detect the...

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Bibliographic Details
Published in:Cancer letters 1995-05, Vol.92 (1), p.67-76
Main Authors: Richmond, R.E., Carter, J.H., Carter, H.W., Daniel, F.B., DeAngelo, A.B.
Format: Article
Language:English
Subjects:
Aldehyde dehydrogenase
Aldehyde Dehydrogenase - analysis
Alpha fetoprotein
alpha-Fetoproteins - analysis
Animals
Biological and medical sciences
Biomarkers, Tumor - analysis
Carcinogenesis, carcinogens and anticarcinogens
Cell Division
Chemical agents
Dichloroacetic acid
Dichloroacetic Acid - toxicity
Experimental hepatocarcinogenesis
Glutathione s-transferase
Glutathione Transferase - analysis
Immunoenzyme Techniques
Liver Neoplasms - chemically induced
Liver Neoplasms - metabolism
Liver Neoplasms - pathology
Male
Medical sciences
Oncogene Protein p21(ras) - analysis
p21 ras
p39 c- jun
p55 c- fos
Proto-Oncogene Proteins c-fos - analysis
Proto-Oncogene Proteins c-jun - analysis
Rats
Rats, Inbred F344
Tumors
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Summary:We examined the incidence of proliferative lesions, hyperplastic nodules and altered hepatic foci, in male F344 rat liver, to determine their preneoplastic potential during dichloroacetic acid (DCA)-induced hepatocarcinogenesis. Immunohistochemical and image analysis methods were used to detect the expression of 6 histochemical markers of neoplastic cells; p21 ras, p39 c- jun, p55 c- fos, aldehyde dehydrogenase (ALDH), glutathione s-transferase (GST-p), and alpha fetoprotein (AFP) during DCA-induced hepatocarcinogenesis. Our results were consistent with our previous data and suggested that the hyperplastic nodules, rather than altered hepatic foci, is a putative preneoplastic lesion during DCA-induced hepatocarcinogenesis in the male F344 rat.
ISSN:0304-3835
1872-7980
DOI:10.1016/0304-3835(94)03756-9