A growth inhibitory factor from lambsquarters (Chenopodium album)

Aqueous extract of air-dried lambsquarters (Chenopodium album) at 25 mg/ml significantly inhibited germination and growth of radish and wheat seeds. Soybean seed germination was not inhibited; however, hypocotyl growth was significantly reduced. Germination of radish seeds in sand amended with pulve...

Full description

Saved in:
Bibliographic Details
Published in:Journal of chemical ecology 1994-04, Vol.20 (4), p.957-967
Main Authors: Mallik, M.A.B, Puchala, R, Grosz, F.A
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
ALELOPATIA
ALLELOPATHIE
ALLELOPATHY
BIOASSAYS
Biological and medical sciences
CHEMICAL COMPOSITION
CHENOPODIUM
Chenopodium album
COMPOSE PHENOLIQUE
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
COMPUESTOS FENOLICOS
CRECIMIENTO
CROISSANCE
DOSAGE BIOLOGIQUE
ENSAYO BIOLOGICO
Fundamental and applied biological sciences. Psychology
Generalities, botany, ecology, damages, economic importance
GLYCINE MAX
GROWTH
INHIBICION
INHIBITION
Parasitic plants. Weeds
PHENOLIC COMPOUNDS
Phytopathology. Animal pests. Plant and forest protection
RAPHANUS SATIVUS
TRITICUM AESTIVUM
Weeds
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Aqueous extract of air-dried lambsquarters (Chenopodium album) at 25 mg/ml significantly inhibited germination and growth of radish and wheat seeds. Soybean seed germination was not inhibited; however, hypocotyl growth was significantly reduced. Germination of radish seeds in sand amended with pulverized lambsquarters shoots at 2 and 4 mg/g was reduced 40 and 95%, respectively. Shoot dry weight and plant height were also reduced 30 and 9%, respectively, at 4 mg/g, but not at 2 mg/g concentration. Residues after extraction with water incorporated in sand were not inhibitory, indicating water solubility of the inhibitor(s). Aqueous extract of shoots decomposed for five days lost nearly 40% of its inhibitory effect; 20% of it still persisted in the extract of shoots decomposed for 30 days. The filtrate from ultrafiltration of aqueous extract through a pad of molecular-weight cutoff 1000 inhibited radish seeds germination and growth, indicating that the molecular weight of the inhibitor(s) was less than 1000. Partitioning of the aqueous extract by a series of solvents resulted in isolation of an inhibitor(s) in the butanol fraction. Seven phenolics were identified in this fraction using high-performance liquid chromatography (HPLC). Paper chromatographic analysis of the butanol fraction revealed six bands, of which one band withR f =0.83 inhibited germination and growth of radish seeds. Chlorogenic acid identified by HPLC appeared to be the principal component of the phytotoxin.
ISSN:0098-0331
1573-1561
DOI:10.1007/bf02059590