Hydrothermal acid treatment for sugar extraction from Golenkinia sp

• Published in: Bioresource technology 2015-08, Vol.190, p.408-411
• Main Authors: Choi, Sun-A, Choi, Won-Il, Lee, Jin-Suk, Kim, Seung Wook, Lee, Gye-An, Yun, Jihyun, Park, Ji-Yeon
• Format: Article
• Language: English
• Subjects: beta-Glucosidase - chemistry; Carbohydrates - chemistry; Carbohydrates - isolation & purification; Cell Fractionation; Cellulase - chemistry; Chlorophyta - chemistry; Enzymatic hydrolysis; Ethanol - chemistry; Ethanol - isolation & purification; Glucose; Golenkinia sp; Hot Temperature; Hydrolysis; Hydrothermal acid treatment; Microalgae - chemistry; Sugar extraction; Sulfuric Acids - chemistry; Water - chemistry
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2015.04.121

[Display omitted] •Hydrothermal acid treatment for sugar extraction from wet microalgae.•The maximal sugar extraction yield at 2.0% sulfuric acid concentration.•An improvement of sugar yield with enzymatic hydrolysis combination. In this study, hydrothermal acid treatment for efficient recovery of sugar from Golenkinia sp. was investigated. The initial glucose and XMG (xylose, mannose, and galactose) contents of a prepared Golenkinia sp. solution (40g/L) were 15.05 and 5.24g/L, respectively. The microalgal cell walls were hydrolyzed, for sugar recovery, by enzymatic saccharification and/or hydrothermal acid treatment. Among the various hydrothermal acid treatment conditions, the most optimal were the 2.0% H2SO4 concentration at 150°C for 15min, under which the glucose- and XMG-extraction yields were 71.7% and 64.9%, respectively. By pH 4.8, 50°C enzymatic hydrolysis after optimal hydrothermal acid treatment, the glucose- and XMG-extraction yields were additionally increased by 8.3% and 0.8%, respectively. After hydrothermal acid treatment, the combination with the enzymatic hydrolysis process improved the total sugar yield of Golenkinia sp. to 75.4%.