Start-up of simultaneous partial nitrification, anammox and denitrification (SNAD) process in sequencing batch biofilm reactor using novel biomass carriers

•Novel carriers used were highly efficient in retaining biomass inside the reactor.•SNAD reactor was started-up in a short period (51d).•Long-term stable operation of SNAD process in SBBR was observed.•SEM analysis confirmed biofilm formation on to the carriers.•qPCR confirmed the enrichment of AOB,...

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Bibliographic Details
Published in:Bioresource technology 2015-08, Vol.190, p.480-486
Main Authors: Daverey, Achlesh, Chen, Yi-Chian, Dutta, Kasturi, Huang, Yu-Tzu, Lin, Jih-Gaw
Format: Article
Language:English
Subjects:
Ammonia
Anammox
Bacteria, Anaerobic - metabolism
Biofilm
Biofilms - growth & development
Bioreactors - microbiology
Carrier
Denitrification
Equipment Design
Equipment Failure Analysis
Nitrites - metabolism
SNAD
Start-up
Water Pollutants, Chemical - isolation & purification
Water Pollutants, Chemical - metabolism
Water Purification - instrumentation
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Summary:•Novel carriers used were highly efficient in retaining biomass inside the reactor.•SNAD reactor was started-up in a short period (51d).•Long-term stable operation of SNAD process in SBBR was observed.•SEM analysis confirmed biofilm formation on to the carriers.•qPCR confirmed the enrichment of AOB, anammox bacteria and denitrifying bacteria. Simultaneous partial nitrification, anammox and denitrification (SNAD) process was started-up in a 2.5L sequencing batch biofilm reactor (SBBR) using novel biomass carriers. The SNAD process took only 51d for start-up at nitrogen loading rate (NLR) and organic loading rate (OLR) of 120 and 60g/m3-d, respectively. Long-term stable operation of SNAD process was observed at NLR and OLR of 360 and 180g/m3-d with average total nitrogen and COD removal efficiencies of >88% and >90%, respectively. The values of conversion ratio Y(NO2-+NO3-)/NH4+ remained below 0.11 after the start-up period, which further confirmed the long-term stability of SNAD process. Results of polymerase chain reaction (PCR), qualitative PCR, and scanning electron microscopic (SEM) analysis of sludge samples confirmed the co-existence and enrichment of AOB, anammox bacteria and denitrifying bacteria in the reactor and biofilm formation on to the carriers.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2015.02.064