Intracerebral antioxidant ability of mature rats after neonatal hypoxic-ischemic brain injury estimated using the microdialysis-electron spin resonance method

• Published in: The journal of obstetrics and gynaecology research 2015-06, Vol.41 (6), p.884-889
• Main Authors: Nakajima, Akira, Ueda, Yuto, Sameshima, Hiroshi, Ikenoue, Tsuyomu
• Format: Article
• Language: English
• Subjects: Animals; antioxidant ability; Antioxidants - analysis; Antioxidants - metabolism; ascorbic acid; Ascorbic Acid - blood; Ascorbic Acid - metabolism; Birth Injuries - blood; Birth Injuries - metabolism; Birth Injuries - physiopathology; Cerebral Cortex - blood supply; Cerebral Cortex - injuries; Cerebral Cortex - metabolism; Cyclic N-Oxides - analysis; Cysteine - blood; Cysteine - metabolism; Disease Models, Animal; Disease Progression; Electron Spin Resonance Spectroscopy; glutathione; Glutathione - blood; Glutathione - metabolism; Humans; hypoxia-ischemia; Hypoxia-Ischemia, Brain - blood; Hypoxia-Ischemia, Brain - metabolism; Hypoxia-Ischemia, Brain - physiopathology; Infant, Newborn; Microdialysis; microdialysis-electron spin resonance method; neonatal rat; Neurons - metabolism; Oxidative Stress; Pyrrolidines - analysis; Rats, Wistar; Spin Labels
• ISSN: 1341-8076; 1447-0756
• DOI: 10.1111/jog.12660

Aim The intracerebral antioxidant ability of mature rats after neonatal hypoxic–ischemic (HI) brain injury was estimated using the microdialysis–electron spin resonance method. Material and Methods Seven‐day‐old Wistar rats were subjected to a modified Levine's procedure for producing HI brain injury. After HI insult, pups were returned and reared with their dams. Seven weeks after HI insult, their intracerebral antioxidant abilities were measured using the microdialysis–electron spin resonance method after the intraperitoneal injection of 3‐methoxycarbonyl‐2,2,5,5‐tetramethylpyrrolidine‐1‐oxyl. Ascorbic acid, L‐cysteine, and glutathione (GSH) were also determined. The rats without HI insult were used as a control. Results The decay rate of 3‐methoxycarbonyl‐2,2,5,5‐tetramethylpyrrolidine‐1‐oxyl in the non‐ligated side of the cerebral hemisphere of the HI group was significantly larger than that of the control group. The amounts of ascorbic acid in the perfusate from the non‐ligated side of the HI group were about four times larger than those of the control group. The amounts of L‐cysteine and GSH of the HI group were about 10 times larger than those of the control group. Conclusions The antioxidant ability in the non‐ligated sides of the cerebral hemispheres of the mature rats 7 weeks after neonatal HI insult was higher than that of the control group. Higher amounts of ascorbic acid and GSH supported the higher antioxidant ability. The increase of the intracerebral antioxidant ability of the non‐ligated side indicates the compensation of motor function for the lost side. The present results should offer important insights into the prognosis for hypoxic–ischemic encephalopathy.