Antioxidant's cytoprotective effects on rotator cuff tenofibroblasts exposed to aminoamide local anesthetics

• Published in: Journal of orthopaedic research 2015-07, Vol.33 (7), p.1001-1007
• Main Authors: Kim, Ra Jeong, Hah, Young-Sool, Kang, Jae-Ran, Park, Hyung Bin
• Format: Article
• Language: English
• Subjects: Anesthetics, Local - adverse effects; Anthocyanins - therapeutic use; antioxidant; Antioxidants - therapeutic use; Caspase 3 - metabolism; Caspase 7 - metabolism; Cells, Cultured; cyanidin; cytotoxicity; Drug Evaluation, Preclinical; Enzyme Activation - drug effects; Fibroblasts - drug effects; Fibroblasts - metabolism; Humans; local anesthetics; Middle Aged; Mitogen-Activated Protein Kinases - drug effects; Poly (ADP-Ribose) Polymerase-1; Poly(ADP-ribose) Polymerases - metabolism; Reactive Oxygen Species - metabolism; Rotator Cuff - drug effects; rotator cuff tenofibroblasts
• ISSN: 0736-0266; 1554-527X
• DOI: 10.1002/jor.22814

ABSTRACT Local anesthetics (LA) are among the drugs most frequently used for musculoskeletal problems, in procedures ranging from diagnosis to postoperative pain control. The cytotoxicity of LA is an emerging area of concern. The purpose of this study was to determine whether cyanidin, an antioxidant, exerts cytoprotective effects against tenofibroblast death induced by LA. Primary cultured human rotator cuff tenofibroblasts were used to evaluate the cytotoxicity of these LA: Ropivacaine (0.075%), Bupivacaine (0.05%), and Lidocaine (0.2%). The effects of cyanidin (100 μg/ml) on the cytotoxicity induced by these LA were investigated. Cell viability, ROS production, caspase‐3/7 activity, and expressions of phospho‐extracellular signal‐regulated kinases (ERK), phospho‐p38, phospho‐c‐Jun N‐terminal kinase (JNK), and cleaved PARP‐1 were evaluated. Exposure to LA significantly induced cell death (p