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Antioxidant's cytoprotective effects on rotator cuff tenofibroblasts exposed to aminoamide local anesthetics
ABSTRACT Local anesthetics (LA) are among the drugs most frequently used for musculoskeletal problems, in procedures ranging from diagnosis to postoperative pain control. The cytotoxicity of LA is an emerging area of concern. The purpose of this study was to determine whether cyanidin, an antioxidan...
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| Published in: | Journal of orthopaedic research 2015-07, Vol.33 (7), p.1001-1007 |
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| container_issue | 7 |
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| container_title | Journal of orthopaedic research |
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| creator | Kim, Ra Jeong Hah, Young-Sool Kang, Jae-Ran Park, Hyung Bin |
| description | ABSTRACT
Local anesthetics (LA) are among the drugs most frequently used for musculoskeletal problems, in procedures ranging from diagnosis to postoperative pain control. The cytotoxicity of LA is an emerging area of concern. The purpose of this study was to determine whether cyanidin, an antioxidant, exerts cytoprotective effects against tenofibroblast death induced by LA. Primary cultured human rotator cuff tenofibroblasts were used to evaluate the cytotoxicity of these LA: Ropivacaine (0.075%), Bupivacaine (0.05%), and Lidocaine (0.2%). The effects of cyanidin (100 μg/ml) on the cytotoxicity induced by these LA were investigated. Cell viability, ROS production, caspase‐3/7 activity, and expressions of phospho‐extracellular signal‐regulated kinases (ERK), phospho‐p38, phospho‐c‐Jun N‐terminal kinase (JNK), and cleaved PARP‐1 were evaluated. Exposure to LA significantly induced cell death (p |
| doi_str_mv | 10.1002/jor.22814 |
| format | article |
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Local anesthetics (LA) are among the drugs most frequently used for musculoskeletal problems, in procedures ranging from diagnosis to postoperative pain control. The cytotoxicity of LA is an emerging area of concern. The purpose of this study was to determine whether cyanidin, an antioxidant, exerts cytoprotective effects against tenofibroblast death induced by LA. Primary cultured human rotator cuff tenofibroblasts were used to evaluate the cytotoxicity of these LA: Ropivacaine (0.075%), Bupivacaine (0.05%), and Lidocaine (0.2%). The effects of cyanidin (100 μg/ml) on the cytotoxicity induced by these LA were investigated. Cell viability, ROS production, caspase‐3/7 activity, and expressions of phospho‐extracellular signal‐regulated kinases (ERK), phospho‐p38, phospho‐c‐Jun N‐terminal kinase (JNK), and cleaved PARP‐1 were evaluated. Exposure to LA significantly induced cell death (p < 0.001), ROS production (p ≤ 0.04), the activation of caspase‐3/7 (p < 0.001), and the increased expressions of phospho‐ERK, phospho‐p38, phospho‐JNK, and cleaved PARP‐1. These LA‐induced cytotoxic effects were reduced by cyanidin. These data indicate that cyanidin, an antioxidant, has cytoprotective effects against LA‐induced cytotoxicity to rotator cuff tenofibroblasts. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:1001–1007, 2015.</description><identifier>ISSN: 0736-0266</identifier><identifier>EISSN: 1554-527X</identifier><identifier>DOI: 10.1002/jor.22814</identifier><identifier>PMID: 25639557</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Anesthetics, Local - adverse effects ; Anthocyanins - therapeutic use ; antioxidant ; Antioxidants - therapeutic use ; Caspase 3 - metabolism ; Caspase 7 - metabolism ; Cells, Cultured ; cyanidin ; cytotoxicity ; Drug Evaluation, Preclinical ; Enzyme Activation - drug effects ; Fibroblasts - drug effects ; Fibroblasts - metabolism ; Humans ; local anesthetics ; Middle Aged ; Mitogen-Activated Protein Kinases - drug effects ; Poly (ADP-Ribose) Polymerase-1 ; Poly(ADP-ribose) Polymerases - metabolism ; Reactive Oxygen Species - metabolism ; Rotator Cuff - drug effects ; rotator cuff tenofibroblasts</subject><ispartof>Journal of orthopaedic research, 2015-07, Vol.33 (7), p.1001-1007</ispartof><rights>2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5254-78a1a8f9e7c49aa79413e26600c9f2080a8554b597b1e66b72c74ba31ba3b1033</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25639557$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Ra Jeong</creatorcontrib><creatorcontrib>Hah, Young-Sool</creatorcontrib><creatorcontrib>Kang, Jae-Ran</creatorcontrib><creatorcontrib>Park, Hyung Bin</creatorcontrib><title>Antioxidant's cytoprotective effects on rotator cuff tenofibroblasts exposed to aminoamide local anesthetics</title><title>Journal of orthopaedic research</title><addtitle>J. Orthop. Res</addtitle><description>ABSTRACT
Local anesthetics (LA) are among the drugs most frequently used for musculoskeletal problems, in procedures ranging from diagnosis to postoperative pain control. The cytotoxicity of LA is an emerging area of concern. The purpose of this study was to determine whether cyanidin, an antioxidant, exerts cytoprotective effects against tenofibroblast death induced by LA. Primary cultured human rotator cuff tenofibroblasts were used to evaluate the cytotoxicity of these LA: Ropivacaine (0.075%), Bupivacaine (0.05%), and Lidocaine (0.2%). The effects of cyanidin (100 μg/ml) on the cytotoxicity induced by these LA were investigated. Cell viability, ROS production, caspase‐3/7 activity, and expressions of phospho‐extracellular signal‐regulated kinases (ERK), phospho‐p38, phospho‐c‐Jun N‐terminal kinase (JNK), and cleaved PARP‐1 were evaluated. Exposure to LA significantly induced cell death (p < 0.001), ROS production (p ≤ 0.04), the activation of caspase‐3/7 (p < 0.001), and the increased expressions of phospho‐ERK, phospho‐p38, phospho‐JNK, and cleaved PARP‐1. These LA‐induced cytotoxic effects were reduced by cyanidin. These data indicate that cyanidin, an antioxidant, has cytoprotective effects against LA‐induced cytotoxicity to rotator cuff tenofibroblasts. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:1001–1007, 2015.</description><subject>Anesthetics, Local - adverse effects</subject><subject>Anthocyanins - therapeutic use</subject><subject>antioxidant</subject><subject>Antioxidants - therapeutic use</subject><subject>Caspase 3 - metabolism</subject><subject>Caspase 7 - metabolism</subject><subject>Cells, Cultured</subject><subject>cyanidin</subject><subject>cytotoxicity</subject><subject>Drug Evaluation, Preclinical</subject><subject>Enzyme Activation - drug effects</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - metabolism</subject><subject>Humans</subject><subject>local anesthetics</subject><subject>Middle Aged</subject><subject>Mitogen-Activated Protein Kinases - drug effects</subject><subject>Poly (ADP-Ribose) Polymerase-1</subject><subject>Poly(ADP-ribose) Polymerases - metabolism</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Rotator Cuff - drug effects</subject><subject>rotator cuff tenofibroblasts</subject><issn>0736-0266</issn><issn>1554-527X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNo9UMtOwzAQtBAISuHADyDf4BLwI46TY1XxVAUC8bpZjrsRLklcYgfav8fQ0oPtkXdmd2cQOqLkjBLCzmeuO2Msp-kWGlAh0kQw-baNBkTyLCEsy_bQvvczQoikLN9Fe0xkvBBCDlA9aoN1CzvVbTjx2CyDm3cugAn2CzBUVUQeuxbHTx1ch01fVThA6ypbdq6stY91WMydhykODuvGti5eU8C1M7rGugUf3iFY4w_QTqVrD4frd4ieLy-extfJ5P7qZjyaJEawuL3MNdV5VYA0aaG1LFLKIbogxBQVIznReTRZikKWFLKslMzItNScxlNSwvkQna76RiuffRyvGusN1HXcxfVe0SwXUlDJaKQer6l92cBUzTvb6G6p_hOKhPMV4dvWsNzUKVG_0asYvfqLXt3eP_6BqEhWCusDLDYK3X2oTHIp1Ovdlcr5y_j2jT8ozn8AjwuG3w</recordid><startdate>201507</startdate><enddate>201507</enddate><creator>Kim, Ra Jeong</creator><creator>Hah, Young-Sool</creator><creator>Kang, Jae-Ran</creator><creator>Park, Hyung Bin</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201507</creationdate><title>Antioxidant's cytoprotective effects on rotator cuff tenofibroblasts exposed to aminoamide local anesthetics</title><author>Kim, Ra Jeong ; Hah, Young-Sool ; Kang, Jae-Ran ; Park, Hyung Bin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5254-78a1a8f9e7c49aa79413e26600c9f2080a8554b597b1e66b72c74ba31ba3b1033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Anesthetics, Local - adverse effects</topic><topic>Anthocyanins - therapeutic use</topic><topic>antioxidant</topic><topic>Antioxidants - therapeutic use</topic><topic>Caspase 3 - metabolism</topic><topic>Caspase 7 - metabolism</topic><topic>Cells, Cultured</topic><topic>cyanidin</topic><topic>cytotoxicity</topic><topic>Drug Evaluation, Preclinical</topic><topic>Enzyme Activation - drug effects</topic><topic>Fibroblasts - drug effects</topic><topic>Fibroblasts - metabolism</topic><topic>Humans</topic><topic>local anesthetics</topic><topic>Middle Aged</topic><topic>Mitogen-Activated Protein Kinases - drug effects</topic><topic>Poly (ADP-Ribose) Polymerase-1</topic><topic>Poly(ADP-ribose) Polymerases - metabolism</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Rotator Cuff - drug effects</topic><topic>rotator cuff tenofibroblasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Ra Jeong</creatorcontrib><creatorcontrib>Hah, Young-Sool</creatorcontrib><creatorcontrib>Kang, Jae-Ran</creatorcontrib><creatorcontrib>Park, Hyung Bin</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of orthopaedic research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Ra Jeong</au><au>Hah, Young-Sool</au><au>Kang, Jae-Ran</au><au>Park, Hyung Bin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antioxidant's cytoprotective effects on rotator cuff tenofibroblasts exposed to aminoamide local anesthetics</atitle><jtitle>Journal of orthopaedic research</jtitle><addtitle>J. Orthop. Res</addtitle><date>2015-07</date><risdate>2015</risdate><volume>33</volume><issue>7</issue><spage>1001</spage><epage>1007</epage><pages>1001-1007</pages><issn>0736-0266</issn><eissn>1554-527X</eissn><abstract>ABSTRACT
Local anesthetics (LA) are among the drugs most frequently used for musculoskeletal problems, in procedures ranging from diagnosis to postoperative pain control. The cytotoxicity of LA is an emerging area of concern. The purpose of this study was to determine whether cyanidin, an antioxidant, exerts cytoprotective effects against tenofibroblast death induced by LA. Primary cultured human rotator cuff tenofibroblasts were used to evaluate the cytotoxicity of these LA: Ropivacaine (0.075%), Bupivacaine (0.05%), and Lidocaine (0.2%). The effects of cyanidin (100 μg/ml) on the cytotoxicity induced by these LA were investigated. Cell viability, ROS production, caspase‐3/7 activity, and expressions of phospho‐extracellular signal‐regulated kinases (ERK), phospho‐p38, phospho‐c‐Jun N‐terminal kinase (JNK), and cleaved PARP‐1 were evaluated. Exposure to LA significantly induced cell death (p < 0.001), ROS production (p ≤ 0.04), the activation of caspase‐3/7 (p < 0.001), and the increased expressions of phospho‐ERK, phospho‐p38, phospho‐JNK, and cleaved PARP‐1. These LA‐induced cytotoxic effects were reduced by cyanidin. These data indicate that cyanidin, an antioxidant, has cytoprotective effects against LA‐induced cytotoxicity to rotator cuff tenofibroblasts. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:1001–1007, 2015.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>25639557</pmid><doi>10.1002/jor.22814</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Anesthetics, Local - adverse effects Anthocyanins - therapeutic use antioxidant Antioxidants - therapeutic use Caspase 3 - metabolism Caspase 7 - metabolism Cells, Cultured cyanidin cytotoxicity Drug Evaluation, Preclinical Enzyme Activation - drug effects Fibroblasts - drug effects Fibroblasts - metabolism Humans local anesthetics Middle Aged Mitogen-Activated Protein Kinases - drug effects Poly (ADP-Ribose) Polymerase-1 Poly(ADP-ribose) Polymerases - metabolism Reactive Oxygen Species - metabolism Rotator Cuff - drug effects rotator cuff tenofibroblasts |
| title | Antioxidant's cytoprotective effects on rotator cuff tenofibroblasts exposed to aminoamide local anesthetics |
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