Molecular features of the sortase enzyme family

Bacteria possess complex and varying cell walls with many surface exposed proteins. Sortases are responsible for the covalent attachment of specific proteins to the peptidoglycan of the cell wall of Gram‐positive bacteria. Sortase A of Staphylococcus aureus, which is seen as the archetypal sortase,...

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Bibliographic Details
Published in:The FEBS journal 2015-06, Vol.282 (11), p.2097-2114
Main Authors: Bradshaw, William J, Davies, Abigail H, Chambers, Christopher J, Roberts, April K, Shone, Clifford C, Acharya, K. Ravi
Format: Article
Language:English
Subjects:
Aminoacyltransferases - antagonists & inhibitors
Aminoacyltransferases - chemistry
Aminoacyltransferases - physiology
Animals
Anti-Bacterial Agents - pharmacology
Anti-Bacterial Agents - therapeutic use
Bacteria - drug effects
Bacteria - enzymology
Bacterial Infections - drug therapy
Bacterial Proteins - antagonists & inhibitors
Bacterial Proteins - chemistry
Bacterial Proteins - physiology
cell walls
crystal structure
Cysteine Endopeptidases - chemistry
Cysteine Endopeptidases - physiology
Enzymes
Gram-positive bacteria
Humans
industrial applications
inhibitor design
Microbiology
Molecular biology
molecular mechanism
pathogenesis
pathogens
peptide recognition
peptidoglycans
Protein Binding
Protein Conformation
proteins
sortase
sortase A
Species Specificity
Staphylococcus aureus
Substrate Specificity
therapeutics
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Summary:Bacteria possess complex and varying cell walls with many surface exposed proteins. Sortases are responsible for the covalent attachment of specific proteins to the peptidoglycan of the cell wall of Gram‐positive bacteria. Sortase A of Staphylococcus aureus, which is seen as the archetypal sortase, has been shown to be essential for pathogenesis and has therefore received much attention as a potential target for novel therapeutics. Being widely present in Gram‐positive bacteria, it is likely that other Gram‐positive pathogens also require sortases for their pathogenesis. Sortases have also been shown to be of significant use in a range of industrial applications. We review current knowledge of the sortase family in terms of their structures, functions and mechanisms and summarize work towards their use as antibacterial targets and microbiological tools.
ISSN:1742-464X
1742-4658
DOI:10.1111/febs.13288