Dual responsive dysprosium-doped hydroxyapatite particles and toxicity reduction after functionalization with folic and glucuronic acids

• Published in: Materials Science & Engineering C 2015-03, Vol.48, p.541-547
• Main Authors: Sánchez Lafarga, Ana Karen, Pacheco Moisés, Fermín P., Gurinov, Andrey, Ortiz, Genaro Gabriel, Carbajal Arízaga, Gregorio Guadalupe
• Format: Article
• Language: English
• Subjects: Animals; Biocompatibility; Biomedical materials; Cations; Confocal; Coprecipitation; Durapatite - adverse effects; Durapatite - chemistry; Durapatite - pharmacokinetics; Durapatite - pharmacology; Dysprosium - adverse effects; Dysprosium - chemistry; Dysprosium - pharmacokinetics; Dysprosium - pharmacology; Fluorescence; Folic Acid - adverse effects; Folic Acid - chemistry; Folic Acid - pharmacokinetics; Folic Acid - pharmacology; Glucuronic Acid - adverse effects; Glucuronic Acid - chemistry; Glucuronic Acid - pharmacokinetics; Glucuronic Acid - pharmacology; Hydroxyapatite; Magnetic Resonance Imaging; Microscopy, Fluorescence; MRI contrast agent; Nanostructure; Oxidative Stress - drug effects; Rats; Rats, Sprague-Dawley; Relaxation time; Toxicity
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2014.12.033

The development of probes for biomedical applications demands materials with low toxicity levels besides fluorescence or magnetic properties to be detected by confocal microscopes or MRI resonators. Several drug delivery systems or other biomedical materials prepared with hydroxyapatite have been proposed, however, toxicity effects might arise when the size of particles is nanometric. In this study, hydroxyapatite functionalized with glucuronic or folic acids presented lower oxidative stress, measured from lipoperoxides and nitric oxide indicators in rats than pure hydroxyapatite. In separated experiments, hydroxyapatite was doped with dysprosium cations by coprecipitation producing a single crystal phase with fluorescent properties easily visualized by confocal microscopy when excited at 488nm. These particles also presented the ability to modify the proton relaxation time in T1 maps collected by magnetic resonance imaging. These modified hydroxyapatite nanoparticles could be candidates to design bimodal probes with low toxicity. [Display omitted] •Hydroxyapatite functionalized with glucuronic acid reduced oxidative stress in rats.•Functionalization with folic acid reduced oxidative stress in rats.•Dysprosium doping does not affect the crystalline structure of hydroxyapatite.•Dysprosium doped particles are visible in fluorescent microscope.•Dysprosium doped particles act as MRI contrast agents.