A CMOS Cantilever-Based Label-Free DNA SoC With Improved Sensitivity for Hepatitis B Virus Detection

This paper presents a highly-integrated DNA detection SoC, where several kinds of cantilever DNA sensors, a readout circuit, an MCU, voltage regulators, and a wireless transceiver, are integrated monolithically in a 0.35 μm CMOS Bio-MEMS process. The cantilever-based biosensors with embedded piezore...

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Bibliographic Details
Published in:IEEE transactions on biomedical circuits and systems 2013-12, Vol.7 (6), p.820-831
Main Authors: Huang, Yu-Jie, Huang, Che-Wei, Lin, Tsung-Hsien, Lin, Chih-Ting, Chen, Li-Guang, Hsiao, Po-Yun, Wu, Bi-Ru, Hsueh, Hsiao-Ting, Kuo, Bing-Jye, Tsai, Hann-Huei, Liao, Hsin-Hao, Juang, Ying-Zong, Wang, Chorng-Kuang, Lu, Shey-Shi
Format: Article
Language:English
Subjects:
Algorithms
Bimedical measurement
Biosensing Techniques - instrumentation
Cantilever
Cantilevers
Circuits
CMOS
CMOS image sensors
Deoxyribonucleic acid
Digital data
DNA
DNA SoC
DNA, Viral - analysis
DNA, Viral - genetics
Frequency variation
Hepatitis
Hepatitis B virus
Hepatitis B virus - genetics
Hepatitis B virus - isolation & purification
Lab-On-A-Chip Devices
label-free
Limit of Detection
Molecular Probe Techniques - instrumentation
oscillator-based
Oscillators
Piezoresistors
Semiconductors
Sensors
System-on-chip
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Summary:This paper presents a highly-integrated DNA detection SoC, where several kinds of cantilever DNA sensors, a readout circuit, an MCU, voltage regulators, and a wireless transceiver, are integrated monolithically in a 0.35 μm CMOS Bio-MEMS process. The cantilever-based biosensors with embedded piezoresistors aim to transduce DNA hybridization into resistance variation without cumbersome labeling process. To improve detection sensitivity for low DNA concentration use, an oscillator-based self-calibrated readout circuit with high precision is proposed to convert small resistance variation ( of original resistance) of the sensor into adequate frequency variation and further into digital data. Moreover, its wireless capacity enables isolation of the sample solution from electrical wire lines and facilitates data transmission. To demonstrate the effectiveness of full system, it is applied to detect hepatitis B virus (HBV) DNA. The experimental results show that it has the capability to distinguish between one base-pair (1-bp) mismatch DNAs and match DNAs and achieves a limit of detection (LOD) of less than 1 pM.
ISSN:1932-4545
1940-9990
DOI:10.1109/TBCAS.2013.2247761