Michelia alba extract attenuates UVB-induced expression of matrix metalloproteinases via MAP kinase pathway in human dermal fibroblasts

• Published in: Food and chemical toxicology 2012-12, Vol.50 (12), p.4260-4269
• Main Authors: Chiang, Hsiu-Mei, Chen, Hsin-Chun, Lin, Tsen-Jung, Shih, I-Chen, Wen, Kuo-Ching
• Format: Article
• Language: English
• Subjects: antioxidant activity; Antioxidants - pharmacology; Biological and medical sciences; Cell Survival - drug effects; Cells, Cultured; collagen; collagenase; Collagens; dermis; Elastase; ERK; extracellular matrix; Fibroblasts; Fibroblasts - cytology; Fibroblasts - drug effects; Fibroblasts - pathology; Fibroblasts - radiation effects; Fluorometry; glycosides; Human; Humans; hyaluronic acid; Hyaluronic Acid - analysis; hydrolysates; hydrolysis; JNK; Kinases; Magnoliaceae - chemistry; MAP Kinase Signaling System; Matrix metalloproteinase; Matrix Metalloproteinase 1 - genetics; Matrix Metalloproteinase 1 - metabolism; Matrix Metalloproteinase 3 - genetics; Matrix Metalloproteinase 3 - metabolism; Matrix Metalloproteinase 9 - genetics; Matrix Metalloproteinase 9 - metabolism; Matrix metalloproteinases; Medical sciences; Michelia; Michelia alba; mitogen-activated protein kinase; p38 Mitogen-Activated Protein Kinases - genetics; p38 Mitogen-Activated Protein Kinases - metabolism; Pathways; Photoaging; Plant Extracts - pharmacology; Polyphenols; Polyphenols - pharmacology; secretion; Signal Transduction; Skin - cytology; Skin - drug effects; Skin - pathology; Skin - radiation effects; Skin Aging - radiation effects; Toxicology; Type I procollagen; Ultraviolet Rays - adverse effects; Up-Regulation - drug effects; Up-Regulation - radiation effects
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2012.08.018

[Display omitted] ► MAE exhibited antioxidant activity. ► MAE inhibited collagenase activity. ► MAE inhibited UVB-induced MMP-1 and MMP-3 expression and MMP-9 activity. ► MAE inhibited UVB-induced ERK and JNK kinase but not p38 kinase expression. ► MAE is an effective agent against UVB-induced photodamage. Solar ultraviolet (UV) radiation can cause skin photoaging by inducing secretion of matrix metalloproteinases (MMPs). It has been reported that MMPs, especially MMP-1, -3 and -9, reduce elasticity of the dermis by degrading collagen. Polyphenols are a group of compounds that exist mainly in glycosides in the plants and they may transform to aglycone after hydrolysis. Polyphenols can inhibit MMP expression and elastase activity. In this study, we investigated the effects of Michelia alba extract (MAE) on expression and activity of MMPs in human skin fibroblast cultures after UVB exposure. The results showed that MAE and its hydrolysates (MAH) inhibited collagenase and elastase activities. In addition, MAE exhibited antioxidant activity, elevated hyaluronic acid content and inhibited UVB-induced MMP-1, MMP-3 and MMP-9 expression. In addition, the zymography assay revealed that MAE also inhibited MMP-9 activity. We also found that MAE inhibited UVB-induced ERK and JNK kinase but not p38 kinase expression, suggesting that MAE may regulate the UVB-induced expression of MMP-1, MMP-3 and MMP-9 via the ERK and JNK kinase pathway. MAE could restore total collagen synthesis reduced by UVB. The results also suggest that MAE treatment may prevent UVB-induced extracellular matrix damage by inhibiting the expression of MMP-1, MMP-3 and MMP-9 through the MAP kinase pathway. Our findings imply that MAE is an effective agent against UVB-induced photodamage.